1887

Abstract

Extracellular 1,3-α--glucan synthase (sucrose: 1,3-α--glucan 3-α--glucosyltransferase, EC 2.4.1. –) of HS6 (serotype ) was purified from culture supernatant by ultrafiltration, DEAE-Sepharose chromatography and preparative isoelectric focusing. The enzyme had a molecular weight of 158000 by SDS-PAGE and an isoelectric point of pH 5·2. The specific activity of the enzyme was 48·3 i.u. (mg protein). The for sucrose was 1·2 m and the activity was optimal at pH 6·0. The enzyme activity was stimulated about 20-fold in the presence of dextran T10. Glucan was synthesized from sucrose by the enzyme and characterized as a linear 1,3-α--glucan by GC–MS.

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1985-03-01
2021-10-19
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