SUMMARY: Extracellular 1,3-α--glucan synthase (sucrose: 1,3-α--glucan 3-α--glucosyltransferase, EC 2.4.1. -) of HS6 (serotype ) was purified from culture supernatant by ultrafiltration, DEAE-Sepharose chromatography and preparative isoelectric focusing. The enzyme had a molecular weight of 158000 by SDS-PAGE and an isoelectric point of pH 5·2. The specific activity of the enzyme was 48·3 i.u. (mg protein). The for sucrose was 1·2 mM and the activity was optimal at pH 6·0. The enzyme activity was stimulated about 20-fold in the presence of dextran T10. Glucan was synthesized from sucrose by the enzyme and characterized as a linear 1,3-α--glucan by GC-MS.


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