1887

Abstract

Five mutants were isolated at the 2 gene on the basis of their inability to utilize hypoxanthine as a sole source of nitrogen. These mutants failed to utilize the purines adenine, hypoxanthine, xanthine, uric acid, allantoin and allantoic acid, although they could utilize urea and ammonium. The 2 mutants appeared to be defective in purine induction of uricase, allantoinase, allantoicase and ureidoglycollase activities but retained wild-type activity of the constitutively synthesized urease. The 2 mutations were recessive.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-3-527
1985-03-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/3/mic-131-3-527.html?itemId=/content/journal/micro/10.1099/00221287-131-3-527&mimeType=html&fmt=ahah

References

  1. Adelberg E., Mandel M., Chen G. C. C. 1965; Optimal conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine in Escherichia coli K12. Biochemical and Biophysical Research Communications 18:788–793
    [Google Scholar]
  2. Cooper R.G. 1980; Selective gene expression and intracellular compartmentation: two means of regulating nitrogen metabolism in yeast. Trends in Biochemical SciencesDecember1–4
    [Google Scholar]
  3. Fluri R., Kinghorn J.R. 1985; The induction of purine catabolism in Schizosaccharomyces pombe. Current Genetics (in the Press)
    [Google Scholar]
  4. Gutz H., Heslot H., Leupold U., Loprieno N. 1974; Schizosaccharomycespombe. In Handbook of Genetics 1395–446 King R. C. New York: Plenum Press;
    [Google Scholar]
  5. Gygax A., Thuriaux P. 1984; A revised chromosome map of the fission yeast Schizosaccharomyces pombe. Current Genetics 8:85–92
    [Google Scholar]
  6. Kinghorn J.R., Fluri R. 1984; Genetic studies of purine breakdown in the fission yeast Schizosaccharomyces pombe. Current Genetics 8:99–105
    [Google Scholar]
  7. Kohli J., Hottinger H., Munz P., Strauss A., Thuriaux P. 1977; Genetic mapping in Schizosaccharomyces pombe by mitotic and meiotic and induced haploidisation. Genetics 87:471–489
    [Google Scholar]
  8. Leupold U. 1970; Genetical methods for Schizo-saccharomyces pombe. Methods in Cell Physiology 4:169–177
    [Google Scholar]
  9. Marzluf G.A. 1981; Regulation of nitrogen metabolism and gene expression in fungi. Microbiological Reviews 45:437–461
    [Google Scholar]
  10. Muftic M.K. 1964; A new phenol-hypochlorate reaction for ammonia. Nature, London 201:622–623
    [Google Scholar]
  11. Scazzocchio C. 1980; The genetics of molybdenum-containing enzymes. In Molybdenum and Molybdenum Containing Enzymes487–516 Coughlan M.P. New York: Pergamon Press;
    [Google Scholar]
  12. Scazzocchio C., Gorton D. 1977; Regulation of purine breakdown. In Genetics and Physiology of Aspergillus255–265 Smith J.E., Pateman J.A. London: Academic Press;
    [Google Scholar]
  13. Sealy-LEWIS H., Scazzocchio C., Lee S. 1978; A mutation defective in the xanthine alternative pathway of Aspergillus nidulans. Its use to investigate the specificity of uaY mediated induction. Molecular and General Genetics 164:303–308
    [Google Scholar]
  14. Snow R. 1966; An enrichment method for auxotrophic yeast mutants using the antibiotic ‘nystatin’. Nature, London 211:206–207
    [Google Scholar]
  15. Vogels G.D., Van Der Drift C. 1979; Differential analysis of glyoxylate derivatives. Analytical Biochemistry 33:143–157
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-3-527
Loading
/content/journal/micro/10.1099/00221287-131-3-527
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error