1887

Abstract

Serovars and of were cultured in the presence of homologous factor serum containing factorial antibodies (FcAbs) to their major antigens. After 39 serial passages they were then re-tested to determine whether their major antigens had remained unchanged. It was found that each parent strain had been replaced by an antigenic variant. The disappearance of each parent strain and its replacement by an antigenic variant was attributed to the selective conditions imposed by FcAbs. The antigenic variants behaved like true mutants. They lacked the major serovar antigens of the parent strains and had acquired some major antigens similar to those of two different serovars, one of which belonged to the same serogroup as the parent strain and the other to a different serogroup.

A comparison of the major antigens of the parent strains with those of their antigenic variants indicated that factorial antibodies may be used selectively to obtain antigenic variants with a predefined pattern of major antigens.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-3-521
1985-03-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/3/mic-131-3-521.html?itemId=/content/journal/micro/10.1099/00221287-131-3-521&mimeType=html&fmt=ahah

References

  1. Addamiano L., Babudieri B. 1955; Ricerche sul comportamento di colture associate di leptospire. Rendiconti dell’Istituto superiore di sanita 18:270–279
    [Google Scholar]
  2. Ananyin V. V., Semynova L. P. 1966; Variability of the serological and antigenic properties of pathogenic leptospires in experimental conditions. Journal of Microbiology, Epidemiology and Immunology 3:34–39
    [Google Scholar]
  3. Babudieri B. 1961; Laboratory diagnosis of lepto-spirosis. Bulletin of the World Health Organization 24:45–58
    [Google Scholar]
  4. Babudieri B. 1968; A new technique for the purification of newly isolated leptospira strains. Bulletin of the World Health Organization 39:939–940
    [Google Scholar]
  5. Babudieri B. 1971; Isolation and study of antigenic mutants of saprophytic and pathogenic leptospires. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene ( Abteilung I, Originate A) 218:75–86
    [Google Scholar]
  6. Babudieri B. 1972; List of Leptospira strains kept in the WHO-FAO Leptospira reference laboratory in Rome. Annali dell’Istituto superiore di sanita 8:159–196
    [Google Scholar]
  7. Bakoss P. 1966; Contribution a la variabilitè de la structure antigènique des leptospires. Annales de la Sociètè beige de mèdicine tropicale 46:97–100
    [Google Scholar]
  8. Bessemans A., Deram R. 1947; Nouvelle transformation antigènique d’une souche leptospirienne. Bulletin de I’Acadèmie royale de mèdicine Belgique 12:236–245
    [Google Scholar]
  9. Bessemans A., Wittebolle P., Niemegers L. 1943; Modification expèrimentale durable de la structure antigènique des leptospires. Bulletin de I’Acadèmie royale de medicine Belgique 8:442–458
    [Google Scholar]
  10. Kmety E. 1960a; Contribution to the antigenic structure of leptospira serotypes forming the Austra-lis serogroup. Journal of Hygiene, Epidemiology, Microbiology and Immunology 4:171–174
    [Google Scholar]
  11. Kmety E. 1960b; An attempt to subdivide the Leptospira Australis A serogroup. In Leptospirae and Leptospirosis in Man and Animals. Wydaw Polskiej Akademie Nauk Warszawa27–39
    [Google Scholar]
  12. Kmety E. 1966; Main antigens as criterion for differentiating leptospiral serotypes. Annales de la Societè beige de mèdicine tropicale 46:103–108
    [Google Scholar]
  13. Kmety E. 1967; Faktorenanalyse von Leptospiren der Icterohaemorrhagiae und einiger werwandter Serogruppen. Biologickè pràce Slovenskej akademie vied 8:3
    [Google Scholar]
  14. Kmety E., Bakoss P. 1964; Beitrag zur Variabilität der Antigenerstruktur der Leptospiren. In II International Symposium on Leptospirae and Lepto-spiroses in Man and Animals123–128 Warzawa: Lublin:
    [Google Scholar]
  15. Kmety E., Galton M. M., Sultzer C. R. 1970; Further standardization of the agglutinin-absorption test in the serology of leptospirosis. Bulletin of the World Health Organization 42:733–738
    [Google Scholar]
  16. Korthoff G. 1932; Experimentelles Schlammfieber beim Menschen. Zentralblattftir Bakteriologie, Para-sitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originate) 125:429–434
    [Google Scholar]
  17. Lataste-Dorolle C., Plesko L. 1969; Analyse factorielle des antigènes leptospiriens (‘Serofacteurs de Kmety’). Bulletin de I’Institut Pasteur 67:2323–2348
    [Google Scholar]
  18. Magliocchetti-Lombi P., Babudieri B. 1968; Ricerche sulle colture associate di leptospira. Annali dell’Istituto superiore di sanità 4:291–304
    [Google Scholar]
  19. Parnas J., Malinowski R., Halgas A. 1961; Experimented Forschungen über die Variability der Leptospiren. Zentralblatt für Bakteriologie und Hygiene (Abteilung I, Originale A) 183:101–118
    [Google Scholar]
  20. Petrov E. M., Cernuckha Y. C. 1977; Potential variability of Leptospira serovars belonging to the same group. Zentralblatt für Bakteriologie und Hygiene (Abteilung I, Originale A) 239:252–261
    [Google Scholar]
  21. Pike R. M., Schultze M. L. 1958; Serologic variants of leptospire types resulting from growth in immune serum. Journal of Immunology 81:172–175
    [Google Scholar]
  22. Silva I. 1974; Studio sistematico di leptospire isolate in Brasile. Annali dell’Istituto superiore di sanita 10:21–25
    [Google Scholar]
  23. Turner L. H. 1968; Leptospirosis. II. Serology. Transactions of the Royal Society of Tropical Medicine and Hygiene 62:880–899
    [Google Scholar]
  24. Yanagawa R., Adachi Y. 1978; Studies of antigenic variants of leptospira isolated from experimentally infected mice. Zentralblatt für Bakteriologie und Hygiene ( Abteilung I, Originale A) 240:347–355
    [Google Scholar]
  25. Yanagawa R., Takashima J. 1974; Conversion of serotype in Leptospira from hebdomadis to kremastos. Infection and Immunity 10:1439–1442
    [Google Scholar]
  26. Yu-En-Shu, Wu Shin-Yi, Lee De-Fen. 1965; Studies on the antagonism between types of lepto-spires and on the method of detecting infections of two mixed types of leptospira. Scientia sinica 14:901–912
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-3-521
Loading
/content/journal/micro/10.1099/00221287-131-3-521
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error