1887

Abstract

Extracellular polymeric material (EP) was isolated from culture supernatants of grown on carbon sources (50 m-glucose, 500 m-sucrose or 500 m-galactose) known to promote yeast adhesion to different extents. Galactose-grown yeasts, which are the most adherent, produced more EP than sucrose-grown organisms, particularly after incubation for 5 d, while glucose-grown yeasts (the least adherent) gave the lowest yield. EP produced on all three carbon sources was of similar composition and contained carbohydrate (65 to 82%; mannose with some glucose), protein (7%), phosphorus (0·5%) and glucosamine (1·5%). Serological studies indicated that these EP preparations were immunologically identical but that galactose-grown yeasts had more antigenic determinants than sucrose-grown organisms while glucose-grown yeasts had the fewest determinants. Antigenic differences were apparent between EP preparations of some strains of . Pretreatment of acrylic strips with EP to form a polymeric coating promoted yeast adhesion to the acrylic surface, but similar pretreatment of buccal epithelial cells with EP inhibited subsequent yeast adhesion. These results indicate that EP originates from the cell surface of and that it contains the surface component(s), probably mannoprotein in nature, responsible for yeast adhesion.

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1985-03-01
2021-10-16
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References

  1. Ashford B. K. 1917; The etiology of sprue. American Journal of Medical Sciences 154:157–176
    [Google Scholar]
  2. Blumerkrantz N., Asboe-Hansen E. 1976; An assay for total hexosamine and a differential assay for glucosamine and galactosamine. Clinical Biochemistry 9:269–274
    [Google Scholar]
  3. Chen P. S., Toribara T. Y., Warner H. 1956; Microdetermination of phosphorus. Analytical Chemistry 28:1756–1758
    [Google Scholar]
  4. Corpe W. A. 1970; An acid polysaccharide produced by a primary film-forming marine bacterium. Developments in Industrial Microbiology 11:402–412
    [Google Scholar]
  5. Douglas L. J., Mccourtie J. 1981; Adherence of Candida albicans to denture acrylic as affected by changes in cell-surface composition. In Current Developments in Yeast Research375–380 Stewart G. G., Russell I. Toronto: Pergamon Press;
    [Google Scholar]
  6. Douglas L. J., Mccourtie J. 1983; Effect of tunicamycin treatment on the adherence of Candida albicans to human buccal epithelial cells. FEMS Microbiology Letters 16:199–202
    [Google Scholar]
  7. Douglas L. J., Houston J. G., Mccourtie J. 1981; Adherence of Candida albicans to human buccal epithelial cells after growth on different carbon sources. FEMS Microbiology Letters 12:241–243
    [Google Scholar]
  8. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  9. Gardiner R., Podgorski C., Day A. W. 1982; Serological studies on the fimbriae of yeasts and yeastlike species. Botanical Gazette 143:534–541
    [Google Scholar]
  10. Hasenclever H. F., Mitchell W. O. 1961; Antigenic studies of Candida. I. Observation of two antigenic groups in Candida albicans . Journal of Bacteriology 82:570–573
    [Google Scholar]
  11. Jeanes A., Wise C. S., Dimler R. J. 1951; Improved techniques in paper chromatography of carbohydrates. Analytical Chemistry 23:415–420
    [Google Scholar]
  12. Jones C. P., Peck R. L. 1940; A green pigment from Candida stellatoidea and Candida albicans . Journal of Bacteriology 39:605–608
    [Google Scholar]
  13. Lee J. C., King R. D. 1983; Characterization of Candida albicans adherence to human vaginal epithelial cells in vitro. Infection and Immunity 41:1024–1030
    [Google Scholar]
  14. Marrie T. J., Costerton J. W. 1981; The ultra-structure of Candida albicans infections. Canadian Journal of Microbiology 27:1156–1164
    [Google Scholar]
  15. Masler L., Šikl D., Bauer Š., Šandula J. 1966; Extracellular polysaccharide-protein complexes produced by selected strains of Candida albicans (Robin) Berkhout. Folia microbiologica 11:373–378
    [Google Scholar]
  16. Mccourtie J., Douglas L. J. 1981; Relationship between cell surface composition of Candida albicans and adherence to acrylic after growth on different carbon sources. Infection and Immunity 32:1234–1241
    [Google Scholar]
  17. Mccourtie J., Douglas L. J. 1984; Relationship between cell surface composition, adherence and virulence of Candida albicans . Infection and Immunity 45:6–12
    [Google Scholar]
  18. Mohamed A-M. H. 1975; Ultrastructural aspects of chronic oral candidosis. Journal of Oral Pathology 4:180–194
    [Google Scholar]
  19. Montes L. F., Wilborn W. H. 1968; Ultrastructural features of host-parasite relationship in oral candidosis. Journal of Bacteriology 96:1349–1356
    [Google Scholar]
  20. Odds F. C. 1979 Candida and Candidosis Leicester: Leicester University Press;
    [Google Scholar]
  21. Ofek I., Beachey E. H. 1980; General concepts and principles of bacterial adherence in animals and man. In Bacterial Adherence, Receptors and Recognition, Series B 61–29 Beachey E. H. London: Chapman & Hall;
    [Google Scholar]
  22. Sandin R. L., Rogers A. L., Patterson R. J., Beneke E. S. 1982; Evidence for mannose-mediated adherence of Candida albicans to human buccal cells in vitro . Infection and Immunity 35:79–85
    [Google Scholar]
  23. Šikl D., Masler L., Bauer Š. 1964; Mannan from the extracellular surface of Candida albicans Berkhout. Experientia 20:456
    [Google Scholar]
  24. Sutherland I. W. 1977; Bacterial exopolysaccharides – their nature and production. In Surface Carbohydrates of the Prokaryotic Cell27–96 Sutherland I. W. London: Academic Press;
    [Google Scholar]
  25. Trevelyan W. E., Proctor D. P., Harrison J. S. 1950; Detection of sugars on paper chromatograms. Nature London: 166444–445
    [Google Scholar]
  26. Wardell J. N., Brown C. M., Flannigan B. 1983; Microbes and surfaces. Symposia of the Society for General Microbiology 34:351–378
    [Google Scholar]
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