A strain of Pseudomonas syringae was isolated from garden soil for its ability to utilize propan-2-yl sulphate as sole source of carbon and energy. Growth was accompanied by disappearance of the ester from culture fluids and formation of stoicheiometric amounts of inorganic sulphate. However, enzyme activity capable of desulphating propan-2-yl sulphate was not detected under a wide range of assay conditions, either intracellular or extracellular, or at different phases of batch culture. No organic radioactive metabolites were detected during growth on propan-2-yl [35S]sulphate or [1, 3-14C]propan-2-yl sulphate alone, but when the latter compound was supplemented with unlabelled lactate, small amounts of 14C-labelled lactate were detected. This and the induction by propan-2-yl sulphate of a specific d-lactate-2-sulphatase led to a proposed pathway in which propan-2-yl sulphate undergoes a stereospecific oxidation to d-lactate-2-sulphate before rapid desulphation to d-lactate and inorganic sulphate.
BarrettC. H.,
DodgsonK. S.,
WhiteG. F.1981; Specificity and other properties of an alcohol dehydrogenase purified from Comamonas terrigena An enzyme exhibiting specificity for l-stereoisomers of secondary alcohols. Biochimica et biophysica acta 661:74–86
BartholomewB.,
DodgsonK. S.,
GorhamS. D.1978; Purification and properties of the S1 secondary alkylsulphohydrolase of the detergent-degrading micro-organism, Pseudomonas C12B. Biochemical Journal 169:659–667
ClovesJ. M.,
DodgsonK. S.,
WhiteG. F.,
FitzgeraldJ. W.1980; Purification and properties of the P2 primary alkylsulphohydrolase of the detergent-degrading bacterium Pseudomonas C12B. Biochemical Journal 185:23–31
CrescenziA. M. V.,
DodgsonK. S.,
WhiteG. F.1984; Purification and some properties of d-lactate-2-sulphatase of Pseudomonas syringae GG. Biochemical Journal 223:487–494
DennerW. H. B.,
OlavesenA. H.,
PowellG. M.,
DodgsonK. S.1969; The metabolism of potassium dodecyl [35S]sulphate in the rat. Biochemical Journal 111:43–51
DodgsonK. S.1961; Determination of inorganic sulphate in studies on the enzymic and non-enzymic hydrolysis of carbohydrate and other sulphate esters. Biochemical Journal 78:312–319
DodgsonK. S.,
WhiteG. F.1983; Some enzymes involved in the microbial degradation of sulphated surfactants. Topics in Enzyme and Fermentation Biotechnology790–155WisemanA.
Chichester: Ellis Horwood;
FitzgeraldJ. W.,
DodgsonK. S.1971a; Sulphur utilization during growth of Pseudomonas fluorescens on potassium d-glucose 6-O-sulphate. Biochemical Journal 121:521–530
FitzgeraldJ. W.,
DodgsonK. S.1971b; Carbon and sulphur utilization during growth of Pseudomonas fluorescens on potassium d-glucose-6-O-sulphate as the sole sulphur source. Biochemical Journal 122:277–283
LloydA. G.,
TudballN.,
DodgsonK. S.1961; Infra red studies on sulphate esters. III. O-Sulphate esters of alcohols, amino alcohols and hydroxylated amino acids. Biochimica et biophysica acta 52:413–419
MaggsJ. L.,
PowellG. M.,
DodgsonK. S.,
HowesD.,
BlackJ. G.,
OlavesenA. H.1982; Metabolism in the rat of potassium dl-octan-2-sulphate, a secondary alkyl sulphate. Xenobiotica 12:101–109
MaggsJ. L.,
PowellG. M.,
OlavesenA. H.,
CurtisC. G.1984; Octan-2-sulphate degradation in the isolated perfused rat liver. Biochemical Pharmacology 33:827–829
MatchamG. W. J.,
DodgsonK. S.,
FitzgeraldJ. W.1977; Purification, properties and cellular localization of the stereospecific CS2 secondary alkylsulphohydrolase of Comamonas terrigena
. Biochemical Journal 167:723–729
ShawD. J.,
DodgsonK. S.,
WhiteG. F.1980; Substrate specificity and other properties of the inducible S3 secondary alkylsulphohydrolase purified from the detergent-degrading bacterium Pseudomonas C12B. Biochemical Journal 187:181–196
WhiteG. F.,
LillisV.,
ShawD. J.1980; An improved procedure for the preparation of alkyl sulphate esters suitable for the study of secondary alkylsulphohydrolase enzymes. Biochemical Journal 187:191–196
WhiteG. F.,
RussellN. J.,
DayM. J.1984; A survey of sodium dodecyl sulphate (SDS) resistance and alkylsulphatase production in bacteria from clean and polluted river sites. Environmental Pollution A37:1–11