1887

Abstract

The serotype-specific capsular polysaccharide from two strains of serotype T4 organisms was purified and characterized by chemical analysis and NMR spectroscopy. The polymer, a teichoic acid, has the backbone structure →(2-glycerol- l)→(phosphate)→(6-α--galactose-l)→ and is partially -acetylated on the C2 and C3 galactose residues. Chemical removal of -acetyl groups from the polysaccharide destroyed both its ability to precipitate with antiserum raised against killed whole serotype T4 organisms and its ability to adhere to sheep erythrocytes in passive haemagglutination experiments. Attempts to elicit antisera using the purified polymer were unsuccessful but a partially purified material was immunogenic.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-2-387
1985-02-01
2021-07-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/2/mic-131-2-387.html?itemId=/content/journal/micro/10.1099/00221287-131-2-387&mimeType=html&fmt=ahah

References

  1. Adlam C., Knights J. M., Mugridge A., Lindon J. C., Baker P. R. W., Beesley J. E., Spacey B., Craig G. R., Nagy L. K. 1984; Purification, characterization and immunological properties of the serotype-specific capsular polysaccharide of Pasteurella haemolytica (serotype Alorganisms. Journal of General Microbiology 130:2415–2426
    [Google Scholar]
  2. Alpenfels W. F. 1981; A rapid and sensitive method for the determination of monosaccharides as their dansyl hydrazones by high-performance liquid chromatography. Analytical Biochemistry 114:153–157
    [Google Scholar]
  3. Bhattacharjee A. K., Jennings H. J., Kenny P., Martin A., Smith I. C. P. 1975; Structural determination of the sialic acid polysaccharide antigens of Neisseria meningitidis serogroups B and C with carbon 13 nuclear magnetic resonance. Journal of Biological Chemistry 250:1926–1932
    [Google Scholar]
  4. Bock K., Thøgerson H. 1982; Nuclear magnetic resonance spectroscopy in the study of mono and polysaccharides. Annual Reports on NMR Spectroscopy 13:1–57
    [Google Scholar]
  5. De Boer W. R., Kruyseen F. J., Wouters J. T. M., Kruk C. 1976; The structure of teichoic acid from Bacillus subtilis var. niger WM as determined by l3C nuclear magnetic-resonance spectroscopy. European Journal of Biochemistry 62:1–6
    [Google Scholar]
  6. De Boer W. R., Wouters J. T. M., Anderson A. J., Archibald A. R. 1978; Further evidence for the structure of the teichoic acids from Bacillus stearo- thermophilus B65 and Bacillus subtilis var. niger WM. European Journal of Biochemistry 85:433–436
    [Google Scholar]
  7. Chen P. S., Toribara T. Y., Warner H. 1956; Microdetermination of phosphorus. Analytical Chemistry 28:1756–1758
    [Google Scholar]
  8. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for the determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  9. Fischer W., Schmidt M. A., Jann B., Jann K. 1982; Structure of the Escherichia coli K2 capsular antigen. Stereochemical configuration of the glycero-phosphate and distribution of galactopyranosyl and residues. Biochemistry 21:1275–1284
    [Google Scholar]
  10. Gilmour N. J. L., Martin W. B., Sharp J. M., Thompson D. A., Wells P. W. 1979; The development of vaccines against pasteurellosis in sheep. Veterinary Record 111:15
    [Google Scholar]
  11. Gotschlich E. C., Rey M., Etienne J., Sanborn W. R., Priau R., Cvjetanovic B. 1972; The immunological responses observed in field studies in Africa with Group A meningococcal vaccine. Progress in Immunological Standardization 5:485–491
    [Google Scholar]
  12. Hestrin S. 1949; The reaction of acetyl choline and other carboxylic acid derivatives with hydroxyl¬amine and its analytical application. Journal of Biological Chemistry 180:249–261
    [Google Scholar]
  13. Jann K., Schmidt M. 1980; Comparative chemical analysis of two variants of the Escherichia coli K2 antigen. FEMS Microbiology Letters 1:79–81
    [Google Scholar]
  14. Jann K., Jann B., Schmidt M. A., Vann W. 1980; Structure of the Escherichia coli K2 capsular antigen, a teichoic acid-like polymer. Journal of Bacteriology 10:1108–1115
    [Google Scholar]
  15. Kurz G., Wallenfels K. 1974; d-Galactose. UV-assay with galactose dehydrogenase. In Methoden der Enzymatischen Analyse, 3. II1279–1282 Bergmeyer. H. U. Stuttgart: Verlag Chemie;
    [Google Scholar]
  16. Lane Smith R., Gilkerson E. 1979; Quantitation of glycosaminoglycan hexosamine using 3- methyl-2-benzothiazolone hydrazone hydrochloride. Analytical Biochemistry 98:478–480
    [Google Scholar]
  17. Michon F., Roy R., Jennings H. J., Ashton F. E. 1984; Structural elucidation of the capsular polysaccharide of Neisseria meningitidis Group H. Canadian Journal of Biochemistry 62:1519–1524
    [Google Scholar]
  18. Mikami T., Nagase T., Matsumoto T., Suzuki S., Suzuki M. 1982; Gelation of Limulus amoebocyte lysate by simple polysaccharides. Microbiology and Immunology 26:403–409
    [Google Scholar]
  19. Nagy L. K., Penn C. W. 1974; Protective antigens in bovine pasteurellosis. Developments in Biological Standardization 26:25–32
    [Google Scholar]
  20. Penn C. W., Nagy L. K. 1976; Isolation of a protective, non toxic capsular antigen from Pasteurella multocida, types B and E.. Research in Veterinary Science 20:90–96
    [Google Scholar]
  21. Peterson G. L. 1979; Review of the Folin phenol protein quantitation method of Lowry, Rosebrough, Farr and Randall. Analytical Biochemistry 100:201–220
    [Google Scholar]
  22. Poxton I. R., Tarelli E., Baddiley J. 1978; The structure of C-polysaccharide from the walls of Streptococcus pneumoniae. Biochemical Journal 175:1033–1042
    [Google Scholar]
  23. Shaskov A. S., Zaretskaya M. S., Yaratosky S. V., Naumova I. B., Chizhov O. S., Shabarova Z. A. 1979; On the structure of the teichoic acid from the cell wall of Streptomyces antibioticus 39. European Journal of Biochemistry 102:477–481
    [Google Scholar]
  24. Spiro R. 1966; Analysis of sugars found in glycoproteins. Methods in Enzymology 8:43–49
    [Google Scholar]
  25. Svennerholm L. 1957; Quantitative estimation of sialic acids II. A colorimetric resorcinol-hydrochloric acid method. Biochimica et biophysica acta 24:604–611
    [Google Scholar]
  26. Van der Kaaden A., Van doorn-van Wakeren J. I. M., Kamerling J. P., Vliegenthart J. F. G., Tiesjema R. H. 1984; Structure of the capsular antigen of Neisseria meningitidis serogroup H. European Journal of Biochemistry 141:513–519
    [Google Scholar]
  27. Wieland O. 1974; Glycerol UV method. In Methoden der Enzymatischen Analyse, 3 rd edn. 111404–1409 Bergmeyer. H. U. Stuttgart: Verlag Chemie;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-2-387
Loading
/content/journal/micro/10.1099/00221287-131-2-387
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error