Summary: We have introduced the hairy root plasmid (pRi) from into . Transfer was accomplished by the use of a Tn5 mutant of the plasmid which had improved transfer abilities. The (pRi) transconjugants did not induce root proliferation in any of the plant species tested, including alfalfa (). The transconjugants nodulated alfalfa and the nodules appeared to be identical, morphologically and cytologically, to those produced by the parent strain. In growth chamber tests, the (pRi) transconjugants induced significantly more nodules per plant on alfalfa than either the parent or the wild-type strain of . The hairy root plasmid was stably maintained in after being passed serially through two plantings of alfalfa.


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