Summary: The composition of membranes from whole cells and minicells of strain BD474 was examined. Two-dimensional electrophoresis showed that the content of several individual polypeptides differed in the two membrane types. Whole cell membranes appeared to contain more penicillin binding protein (PBP) 2a. glycerol-3-phosphate dehydrogenase, succinate dehydrogenase and NADH dehydrogenase than minicell membranes, but less PBP2b, lactate dehydrogenase and malate dehydrogenase. Glycerol-3-phosphate dehydrogenase and succinate dehydrogenase were unstable, but the other enzymes and PBPs were stable after inhibition of protein synthesis by chloramphenicol. Minicells synthesized peptidoglycan because they incorporated C-labelled acetylglucosamine into acid precipitable material. However, the rate of incorporation in minicells was only 5% of the value observed in whole cells. Whole cell and minicell membranes contained the same polar lipids and fatty acids, but the whole cell membranes were enriched in phosphatidylglycerol, diglycosyldiacylglycerol, -C and -C fatty acids, but depleted of phosphatidylethanolamine, -C, -C, -C and -C fatty acids.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error