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The accumulation of 4-desmethyl and 4,4-dimethyl sterols, as well as the triterpenoid β-amyrin, was analysed during both exponential and stationary phases of Aspergillus nidulans growth. Throughout growth, the amount of 4-desmethyl sterol was proportional to the cellular dry weight, while the dimethyl sterols and β-amyrin stopped accumulating after day 2. The sterols were found primarily as the free alcohol and not as fatty acid esters, the glycosides, or acyl glycosides. The amount of β-amyrin in stationary phase cultures was affected by the concentrations of Mg2+and Cu2+.
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