The Effect of Nickel on Hydrogen Metabolism and Nitrogen Fixation in the Cyanobacterium Free

Abstract

A comparative study was made of the growth and nitrogen fixation of nickel-depleted and nickel-supplemented cultures of the cyanobacterium . Four sets of growth conditions were used, involving both dark/light and continuous light regimes, anaerobic and aerobic conditions, light limitation and supplementation of the gas phase with hydrogen. In each case nickel-containing cells had an active hydrogen uptake capacity whereas nickel-depleted cells did not. These differences in hydrogenase activities did not correlate with differences in acetylene reduction and growth rates, or fixed nitrogen, phycocyanin or chlorophyll contents. It is concluded that under the growth conditions used the capacity of cells to consume hydrogen gas confers no advantage to the organisms in terms of their growth rates and nitrogen fixation.

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1985-02-01
2024-03-29
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References

  1. Allen M. B., Arnon D. I. 1955; Studies on nitrogen-fixing blue-green algae I. Growth and nitrogen fixation by Anabaena cylindrica Lemm. Plant Physiology 30:366–372
    [Google Scholar]
  2. Babich H., Stotzky G. 1983; Toxicity of nickel to microbes : environmental aspects. Advances in Applied Microbiology 29:195–265
    [Google Scholar]
  3. Bergersen F. J. 1980; Measurement of nitrogen fixation by direct means. In Methods for Evaluating Biological Nitrogen Fixation66–76 Bergersen F. J. New York: John Wiley & Sons;
    [Google Scholar]
  4. Berns D. S., Holohan P., Scott E. 1966; Urease activity in blue-green algae. Science 152:1077–1078
    [Google Scholar]
  5. Bothe H., Distler E., Eisbrenner G. 1978; Hydrogen metabolism in blue-green algae. Biochimie 60:277–289
    [Google Scholar]
  6. Craig J. W., Carr N. G. 1968; C-Phycocyanin and allophycocyanin in two species of blue-green alga. Biochemical Journal 106:361 366:
    [Google Scholar]
  7. Daday A., Smith G. D. 1983; The effect of nickel on the hydrogen metabolism of the cyanobacterium Anabaena cylindrica. FEMS Microbiology Letters 20:327–330
    [Google Scholar]
  8. Daday A., Platz R. A., Smith G. D. 1977; Anaerobic and aerobic hydrogen gas formation by the blue-green alga Anabaena cylindrica. Applied and Environmental Microbiology 34:478–483
    [Google Scholar]
  9. Dixon R. O. D. 1972; Hydrogenase in legume root nodule bacteroids: occurrence and properties. Archives of Microbiology 85:193–201
    [Google Scholar]
  10. Eisbrenner G., Evans H. J. 1983; Aspects of hydrogen metabolism in nitrogen-fixing legumes and other plant-microbe associations. Annual Review of Plant Physiology 34:105–136
    [Google Scholar]
  11. Eisbrenner G., Roos P., Bothe H. 1981; The number of hydrogenases in cyanobacteria. Journal of General Microbiology 125:383–390
    [Google Scholar]
  12. Eskew D. L., Welch R. M., Cary E. E. 1983; Nickel: an essential micronutrient for legumes and possibly all higher plants. Science 222:621–623
    [Google Scholar]
  13. Evans H. J., Purohit K., Cantrell M. A., Eisbrenner G., Russel S. A., Hanus F. J., Lepo J. E. 1981; Hydrogen losses and hydrogenases in nitrogen-fixing organisms. In Current Perspectives in Nitrogen Fixation84–96 Gibson A. H., Newton. W. E. Canberra: Australian Academy of Science;
    [Google Scholar]
  14. Guth J. H., Burris R. H. 1983; Inhibition of nitrogenase-catalyzed NH3 formation by H2. Biochemistry 22:5111–5122
    [Google Scholar]
  15. Houchins J. P., Burris R. H. 1981a; Light and dark reactions of the uptake hydrogenase in Anabaena 7120. Plant Physiology 68:712–716
    [Google Scholar]
  16. Houchins J. P., Burris R. H. 1981b; Physiological reactions of the reversible hydrogenase from Anabaena 7120. Plant Physiology 68:717–721
    [Google Scholar]
  17. Kessler E. 1973; Effect of anaerobiosis on photosyn-thetic reactions and nitrogen metabolism of algae with and without hydrogenase. Archives of Microbiology 93:91–100
    [Google Scholar]
  18. Kessler E. 1974; Hydrogenase photoreduction and anaerobic growth. In Algal Physiology and Biochemistry456–473 Stewart. W. D. P. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  19. Klucas R. V., Hanus F. J., Russell S. A., Evans H. J. 1983; Nickel: a micronutrient element for hydrogen-dependent growth of Rhizobium japonicum and for expression of urease activity in soybean leaves. Proceedings of the National Academy of Sciences of the United States of America 802253–2257
    [Google Scholar]
  20. Laczkó I., Barabás K. 1981; Hydrogen evolution by photobleached Anabaena cylindrica. Planta 153:312–316
    [Google Scholar]
  21. Lambert G. R., Smith G. D. 1980; Hydrogen metabolism by filamentous cyanobacteria. Archives of Biochemistry and Biophysics 205:36–50
    [Google Scholar]
  22. Lambert G. R., Smith G. D. 1981; The hydrogen metabolism of cyanobacteria. Biological Reviews 56:589–660
    [Google Scholar]
  23. Mallette M. F. 1969; Evaluation of growth by physical and chemical means. Methods in Microbiology 1:521–566
    [Google Scholar]
  24. Mortenson L. E. 1978; The role of dihydrogen and hydrogenase in nitrogen fixation. Biochimie 60:219223
    [Google Scholar]
  25. Neilson A., Rippka R., Kunisawa R. 1971; Heterocyst formation and nitrogenase synthesis in Anabaena sp. Archives of Microbiology 76:139–150
    [Google Scholar]
  26. Phillips D. 1980; Efficiency of symbiotic nitrogen fixation in legumes. Annual Review of Plant Physiology 31:29–49
    [Google Scholar]
  27. Postgate J. 1978; Nitrogen Fixation. 3 Studies in Biology 92: London: Edward Arnold;
    [Google Scholar]
  28. Rainbird R. M., Atkins C. A., Pate J. S., Sanford P. 1983; Significance of hydrogen evolution in the carbon and nitrogen economy of nodulated cowpea. Plant Physiology 71:122–127
    [Google Scholar]
  29. Salminen S. O., Nelson L. M. 1984; Role of uptake hydrogenase in providing reductant for nitrogenase in Rhizobium leguminosarum bacteroids. Biochimica et biophysica acta 764:132–137
    [Google Scholar]
  30. Spencer D. F., Greene R. W. 1981; Effects of nickel on seven species of freshwater algae. Environmental Pollution, Series A 25:241–247
    [Google Scholar]
  31. Stewart W. D. P. 1980; Transport and utilization of nitrogen sources by algae. In Microorganisms and Nitrogen Sources. Transport and Utilization of Amino Acids, Peptides, Proteins and Related Substances577–607 Payne. J. W. New York: John Wiley & Sons;
    [Google Scholar]
  32. Thauer R. K. . 1983; Three new nickel enzymes from anaerobic bacteria. Naturwissenschaften 70:60–64
    [Google Scholar]
  33. Thiers R. E. 1957; Contamination in trace element analysis and its control. Methods of Biochemical Analysis 5:273–335
    [Google Scholar]
  34. Van Baalen C., O’Donnell R. 1978; Isolation of a nickel-dependent blue-green alga. Journal of General Microbiology 105:351–353
    [Google Scholar]
  35. Weissman J. C., Benemann J. R. 1977; Hydrogen production by nitrogen-starved cultures of Anabaena cylindrica. Applied and Environmental Microbiology 33:123–131
    [Google Scholar]
  36. Welch R. M. 1981; The biological significance of nickel. Journal of Plant Nutrition 3:345–356
    [Google Scholar]
  37. Wolin M. J. 1982; Hydrogen transfer in microbial communities. Microbial Interactions and Communities 1:323–356
    [Google Scholar]
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