1887

Abstract

Summary: The non-conjugative plasmid pAV5 specifies resistance to kanamycin/neomycin (Km) and tetracycline (Tc). Physical evidence is presented to show that pAV5 gives rise to two plasmids, pAV51 (Km) and pAV52 (Tc), which are formed by deletion of apparently non-overlapping segments of pAV5. Expression of Tc has been obtained in and is associated with a 1.9 kb III fragment found in pAV5 and in pAV52. Expression of Km has been obtained in and is associated with a 1.3 kb I fragment found in pAV5 and pAV51. Evidence is presented that the Km gene is flanked by inverted repeat sequences and is therefore tentatively identified as a transposon, designated Tn. The Km gene specifies an aminoglycoside 3'-phosphotransferase-type I (APH(3')-I) enzyme.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-12-3367
1985-12-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/12/mic-131-12-3367.html?itemId=/content/journal/micro/10.1099/00221287-131-12-3367&mimeType=html&fmt=ahah

References

  1. Berg D. E., Jorgensen R., Davies J. 1978; Transposable kanamycin-neomycin resistance determinants.. In Microbiology – 1978 pp 13–15 Edited by Schlessinger D. Washington: American Society for Microbiology;
    [Google Scholar]
  2. Brack C. 1981; DNA electron microscopy.. Critical Reviews in Biochemistry 10:114–169
    [Google Scholar]
  3. Chopade B. A., Wise P. J., Towner K. J. 1985; Plasmid transfer and behaviour in Acinetobacter calcoaceticus EBF 65/65.. Journal of General Microbiology 131:2805–2811
    [Google Scholar]
  4. Clewell D. B., Helinski D. R. 1969; Supercoiled circular protein–DNA complex in Escherichia coli: purification and induced conversion to an open circular DNA form.. Proceedings of the National Academy of Sciences of the United States of America 62:1159–1166
    [Google Scholar]
  5. Cruze J. A., Singer J. T., Finnerty W. R. 1979; Conditions for quantitative transformation in Acinetobacter calcoaceticus . Current Microbiology 3:129–132
    [Google Scholar]
  6. Daniels D. L., de Wet J. R., Blattner F. R. 1980; New map of bacteriophage lambda DNA.. Journal of Virology 33:390–400
    [Google Scholar]
  7. Devaud M., Kayser F. H., Bächi B. 1982; Transposon-mediated multiple antibiotic resistance in Acinetobacter strains.. Antimicrobial Agents and Chemotherapy 22:323–329
    [Google Scholar]
  8. Dibb W., Kjellvold V. A., Digranes A. 1983; Pseudomonas aeruginosa and Acinetobacter calcoaceticus in vitro susceptibility of 150 clinical isolates to 5β-lactam antibiotics and tobramycin.. Chemotherapy 29:332–336
    [Google Scholar]
  9. Divers M., Hinchliffe E., Vivian A. 1984; Characterisation of an antibiotic resistance plasmid, pAV5 and its constituent replicons in Acinetobacter calcoaceticus . Genetical Research 44:1–9
    [Google Scholar]
  10. Ferguson J., Davis R. W. 1978; Quantitative electron microscopy of nucleic acids.. In Advanced Techniques in Biological Electron Microscopy vol II pp 123–171 Edited by Koehler J. K. Berlin: Springer-Verlag;
    [Google Scholar]
  11. French G. L., Casewell M. W., Roncoroni A. J., Knight S. I., Phillips I. 1980; A hospital outbreak of antibiotic-resistant Acinetobacter anitratus: epidemiology and control.. Journal of Hospital Infection 1:125–131
    [Google Scholar]
  12. Goldstein F. W., Labigne-Roussel A., Gerbaud G., Carlier C., Collatz E., Courvalin P. 1983; Transferable plasmid-mediated antibiotic resistance in Acinetobacter . Plasmid 10:138–147
    [Google Scholar]
  13. Grindley N. D. F., Joyce C. M. 1980; Genetic and DNA sequence analysis of the kanamycin resistance transposon Tn903 . Proceedings of the National Academy of Sciences of the United States of America 77:7176–7180
    [Google Scholar]
  14. Grunstein M., Hogness D. S. 1975; Colony hybridisation: a method for the isolation of cloned DNAs that contain a specific gene.. Proceedings of the National Academy of Sciences of the United States of America 72:3961–3965
    [Google Scholar]
  15. Hershfield V., Boyer H. W., Chow L., Helinski D. R. 1976; Characterisation of a mini Col E1 plasmid.. Journal of Bacteriology 126:447–453
    [Google Scholar]
  16. Hinchliffe E., Vivian A. 1980a; Naturally occurring plasmids in Acinetobacter calcoaceticus: a P class R factor of restricted host range.. Journal of General Microbiology 116:75–80
    [Google Scholar]
  17. Hinchliffe E., Vivian A. 1980b; Gene transfer in Acinetobacter calcoaceticus: fertility variants of the sex factor pAV1.. Journal of General Microbiology 119:117–122
    [Google Scholar]
  18. Hinchliffe E., Nugent M. E., Vivian A. 1980; Naturally occurring plasmids in Acinetobacter calcoaceticus: pAV2, a plasmid which influences the fertility of the sex factor pAV1.. Journal of General Microbiology 121:411–418
    [Google Scholar]
  19. Kleckner N. 1981; Transposable genetic elements.. Annual Review of Genetics 15:341–404
    [Google Scholar]
  20. Kushner S. R. 1978; An improved method for transformation of Escherichia coli with Col E1-derived plasmids.. In Genetic Engineering pp 17–23 Edited by Boyer H. B., Nicosia S. Amsterdam: Elsevier;
    [Google Scholar]
  21. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Murray B. E., Moellering R. C. Jr 1980; Evidence of plasmid-mediated production of aminoglycoside-modifying enzymes not previously described in Acinetobacter . Antimicrobial Agents and Chemotherapy 17:30–36
    [Google Scholar]
  23. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  24. Sanger F., Coulson A. R., Friedman T., Air G. M., Barrell B. G., Brown N. L., Fiddes J. C., Hutchinson C. A., Slocombe P. M., Smith M. 1978; The nucleotide sequence of bacteriophage ΦX174.. Journal of Molecular Biology 125:225–246
    [Google Scholar]
  25. Shimizu S., Inoue M., Mitsuhashi S. 1981; Enzymatic adenylation of spectinomycin by Acinetobacter calcoaceticus subsp. anitratus . Journal of Antibiotics 34:869–875
    [Google Scholar]
  26. Soberon X., Covarrubias L., Bolivar F. 1980; Construction and characterisation of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325.. Gene 9:287–305
    [Google Scholar]
  27. Stuber D., Bujard H. 1977; Electron microscopy of DNA: determination of absolute molecular weight and linear density.. Molecular and General Genetics 154:299–303
    [Google Scholar]
  28. Towner K. J., Vivian A. 1976; RP4 fertility variants in Acinetobacter calcoaceticus . Genetical Research 28:301–306
    [Google Scholar]
  29. Twigg A. J., Sherratt D. J. 1980; Transcomplementable copy number mutants of plasmid Col E1.. Nature, London 283:216–218
    [Google Scholar]
  30. Vieira J., Messing J. 1982; The pUC plasmids, an M13 mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.. Gene 19:259–268
    [Google Scholar]
  31. Vivian A., Hinchliffe E., Fewson C. A. 1981; Acinetobacter calcoaceticus: some approaches to a problem.. Journal of Hospital Infection 2:199–203
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-12-3367
Loading
/content/journal/micro/10.1099/00221287-131-12-3367
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error