Summary: An extracellular glucosyltransferase (sucrose: 1,6-, 1,3-α-D-glucan 3-α- and 6-α-D-glucosyltransferase, EC 2.4.1.-) of HS6 (serotype ) was purified from culture supernatant by DEAE-Sepharose chromatography and preparative isoelectric focusing. The molecular weight measured by SDS-PAGE was 159000 and the isoelectric point was pH 4.9. The specific activity was 89.7 i.u. (mg protein) and the optimum pH was 6.0. The value for sucrose was 4.9 mM and the enzyme activity was not stimulated by exogenous dextran T10. Glucan was synthesized from sucrose by the purified enzyme and consisted of 49.1 mol% 1,6-α-linked glucose and 33.9 mol% 1,3-α-linked glucose, with 13.6 mol% terminal glucose and 3.3 mol% 1,3,6-α-branched glucose.


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