1887

Abstract

Summary: A gene for the β-lactamase from alkalophilic sp. strain 170 was cloned in a functional state on a 1-0 kb DNA fragment and its nucleotide sequence was determined. The coding sequence showed an open reading frame of 257 amino acids, which represents the β-lactamase precursor protein. It is considered that the signal peptide consisted of 30 amino acids including 12 hydrophobic amino acids.

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/content/journal/micro/10.1099/00221287-131-12-3317
1985-12-01
2021-10-28
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References

  1. Ambler R. P. 1980; The structure of β-lactamases.. Philosophical Transactions of the Royal Society of London B289:321–331
    [Google Scholar]
  2. Blin N., Stafford D. W. 1976; A general method for isolation of high molecular weight DNA from eukaryotes.. Nucleic Acids Research 3:2303–2308
    [Google Scholar]
  3. Boliver F., Rodoriguez R. L., Greene P. J., Betlach M. C., Heynecker H. L., Boyer H. W. 1977; Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.. Gene 2:95–113
    [Google Scholar]
  4. Edman P., Henschen A. 1975; Sequence determination.. In Protein Sequence Determination 2nd edn, pp 232–279 Edited by Needleman S. B. Berlin: Springer–Verlag;
    [Google Scholar]
  5. Goldfarb D. S., Rodoriguez R. L., Doi R. H. 1982; Translation block to expression of the Escherichia coli Tn-9 derived chloramphenicol-resistance gene in Bacillus subtilis . Proceedings of the National Academy of Sciences of the United States of America 79:5886–5890
    [Google Scholar]
  6. Hershfield V., Boyer H. W., Yanofsky C., Lovett W. A., Helinsky D. R. 1974; Plasmid ColE1 as a molecular vehicle for cloning and amplification of DNA.. Proceedings of the National Academy of Sciences of the United States of America 71:3455–3459
    [Google Scholar]
  7. Izui K., Nielsen J. B. K., Caulfield M. P., Lampen J. O. 1980; Large exopenicillinase, initial extracellular form detected in cultures of Bacillus licheniformis . Biochemistry 19:1882–1886
    [Google Scholar]
  8. Kato C., Kudo T., Watanabe K., Horikoshi K. 1983; Extracellular production of Bacillus penicillinase by Escherichia coli carrying pEAP2.. European Journal of Applied Microbiology and Biotechnology 18:339–343
    [Google Scholar]
  9. Kato C., Kudo T., Horikoshi K. 1984; Gene expression and production of Bacillus No. 170 penicillinase in Escherichia coli and Bacillus subtilis . Agricultural and Biological Chemistry 48:397–401
    [Google Scholar]
  10. Kreil G. 1981; Transfer of proteins across membranes.. Annual Review of Biochemistry 50:317–348
    [Google Scholar]
  11. Kroyer J., Chang S. 1981; The promoter-proximal region of the Bacillus licheniformis penicillinase gene: nucleotide sequence and predicted leader peptide sequence.. Gene 15:343–347
    [Google Scholar]
  12. Kudo T., Kato C., Horikoshi K. 1983; Excretion of the penicillinase of an alkalophilic Bacillus sp. through the Escherichia coli outer membrane.. Journal of Bacteriology 156:949–951
    [Google Scholar]
  13. Kudo T., Yoshitake J., Kato C., Usami R., Horikoshi K. 1985; Cloning of a developmentally regulated element from alkalophilic Bacillus subtilis DNA.. Journal of Bacteriology 161:158–163
    [Google Scholar]
  14. Lai J. -S., Sarvas M., Brammer W. J., Neugebauer K., Wu H. 1981; Bacillus licheniformis penicillinase synthesized in Escherichia coli contains covalently linked fatty acid and glyceride.. Proceedings of the National Academy of Sciences of the United States of America 78:3506–3510
    [Google Scholar]
  15. Lawn R. M., Adelman J., Franke A. E., Houck C. M., Gross M., Najarian R., Goeddel D. V. 1981; Human fibroblast interferon gene lacks introns.. Nucleic Acids Research 9:1045–1052
    [Google Scholar]
  16. Messing J. 1981 In Third Cleveland Symposium on Macromolecules: Recombinant DNA pp 143–153 Edited by Walton A. Amsterdam: Elsevier;
    [Google Scholar]
  17. Mezes P. S. F., Yang Y. Q., Hussain M., Lampen O. 1983; Bacillus cereus 569/H β-lactamase I: cloning in Escherichia coli and signal sequence determination.. FEBS Letters 161:195–200
    [Google Scholar]
  18. Neugebauer K., Sprengel R., Schaller H. 1981; Penicillinase from Bacillus licheniformis: nucleotide sequence of the gene and implications for the biosynthesis of a secretory protein in a Gram-positive bacterium.. Nucleic Acids Research 9:2577–2588
    [Google Scholar]
  19. Nielsen J. B. K., Lampen J. O. 1982; Membrane bound penicillinases in gram-positive bacteria.. Journal of Bacteriology 257:4490–4495
    [Google Scholar]
  20. Rosenberg M., Court D. 1979; Regulatory sequences involved in the promotion and termination of RNA transcription.. Annual Review of Genetics 13:319–353
    [Google Scholar]
  21. Sancar A., Hack A. M., Rupp W. D. 1979; Simple method for identification of plasmid-coded proteins.. Journal of Bacteriology 137:692–693
    [Google Scholar]
  22. Sancar A., Rupert C. S. 1978; Determination of plasmid molecular weights from ultraviolet sensitivities.. Nature, London 272:471–472
    [Google Scholar]
  23. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequence with chain-terminating inhibitors.. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  24. Sashihara N., Kudo T., Horikoshi K. 1984; Molecular cloning and expression of cellulase genes of alkalophilic Bacillus sp. strain N-4 in Escherichia coli . Journal of Bacteriology 158:503–506
    [Google Scholar]
  25. Simons K., Sarvas M., Garoff H., Helenius A. 1978; Membrane bound and secreted forms of penicillinase from Bacillus licheniformis . Journal of Molecular Biology 126:673–690
    [Google Scholar]
  26. Sloma A., Gross M. 1983; Molecular cloning and nucleotide sequence of the type I β-lactamase gene from Bacillus cereus . Nucleic Acids Research 11:4997–5004
    [Google Scholar]
  27. Sunaga T., Akiba T., Horikoshi K. 1976; Production of penicillinase by an alkalophilic Bacillus . Agricultural and Biological Chemistry 40:1363–1367
    [Google Scholar]
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