1887

Abstract

Summary: Protoplasts were formed from aged germlings and mycelia of , using a combination of chitinase and β-glucanase. Methanol, ethanol and propanol stimulated protoplast formation, apparently by a mechanism different from that of mercaptoethanol. Regeneration of protoplasts in soft agar was detectable by light microscopy 2 h after incubation and colonies were observable by the naked eye within 40 h; the regeneration rate was 65%.

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/content/journal/micro/10.1099/00221287-131-12-3311
1985-12-01
2021-10-20
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