Summary: Polar lipid patterns of representative methanogens were recorded by two-dimensional thin-layer chromatography. Phenotypically similar spp., spp. and spp. could readily be distinguished from each other. Similarly, spp. and phenotypically similar spp. had different polar lipid patterns. Single examples from the monospecific genera and had distinctive polar lipid patterns, but had a similar pattern to spp. The isopranoid ether lipid cores from the polar lipids were identical for those species within any one genus. Novel core lipids were identified in examples from the genera and


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