RT Journal Article SR Electronic(1) A1 Lebens, Michael R. A1 Williams, Peter A.YR 1985 T1 Complementation of Deletion and Insertion Mutants of TOL Plasmid pWW0: Regulatory Implications and Location of xylC Gene JF Microbiology, VO 131 IS 12 SP 3261 OP 3269 DO https://doi.org/10.1099/00221287-131-12-3261 PB Microbiology Society, SN 1465-2080, AB Summary: Two spontaneous mutant plasmids of TOL plasmid pWW0 are described. pWW0-660 contains an 18 kbp deletion of the entire xylCAB operon and pWW0-673 has a 3.4 kbp insert in the operator-promoter (OP1) region of xylCAB. In Pseudomonas putida hosts containing either plasmid, m-xylene was unable to act as a growth substrate or as an inducer of enzymes of the xylCAB or of the xylDLEGF operons. Complementation of these strains with a recombinant plasmid pWW0-3006 carrying the HindIII region D (HD) of pWW0 restored the Mxy+ phenotype and the ability of m-xylene to induce the xylDLEGF operon. The cloned HD region had no regulatory function but carried the OP1 region, the entire xylC structural gene and sufficient of xylA to support growth on m-xylene. XylC was more precisely located on a 1.9 kbp Sa/I subclone of the HD region. Comparison of the roles of m-xylene and m-methylbenzyl alcohol as inducers shows they are not equivalent and that whereas m-xylene can induce only xylCAB, m-methylbenzyl alcohol can induce both xylCAB and xylDLEGF. The restoration of the induction of xylDLEGF by m-xylene is due to the presence of xylA on the recombinant pWW0-3006, converting m-xylene to m-methylbenzyl alcohol., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-131-12-3261