1887

Abstract

Summary: Two spontaneous mutant plasmids of TOL plasmid pWW0 are described. pWW0-660 contains an 18 kbp deletion of the entire operon and pWW0-673 has a 3.4 kbp insert in the operator-promoter (OP1) region of . In hosts containing either plasmid, -xylene was unable to act as a growth substrate or as an inducer of enzymes of the or of the operons. Complementation of these strains with a recombinant plasmid pWW0-3006 carrying the III region D (HD) of pWW0 restored the Mxy phenotype and the ability of -xylene to induce the operon. The cloned HD region had no regulatory function but carried the OP1 region, the entire structural gene and sufficient of to support growth on -xylene. was more precisely located on a 1.9 kbp /I subclone of the HD region. Comparison of the roles of -xylene and -methylbenzyl alcohol as inducers shows they are not equivalent and that whereas -xylene can induce only -methylbenzyl alcohol can induce both and . The restoration of the induction of by -xylene is due to the presence of on the recombinant pWW0-3006, converting -xylene to -methylbenzyl alcohol.

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/content/journal/micro/10.1099/00221287-131-12-3261
1985-12-01
2021-07-31
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References

  1. Bagdasarian M., Lurz R., Ruckert B., Franklin F. C. H., Bagdasarian M. M., Frey J., Timmis K. N. 1981; Specific purpose cloning vectors. II. Broad host-range, high copy-number RSF1010-derived vectors and a host vector system for gene cloning in Pseudomonas . Gene 16:237–247
    [Google Scholar]
  2. Bolivar F. 1978; Construction and characterisation of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique EcoRI sites for selection of EcoRI generated recombinant DNA molecules.. Gene 4:121–136
    [Google Scholar]
  3. Franklin F. C. H., Williams P A. 1980; Construction of a partial diploid for the degradative pathway encoded by the TOL plasmid (pWW0) from Pseudomonas putida mt-2: evidence for the positive nature of the regulation of xylR gene.. Molecular and General Genetics 177:321–328
    [Google Scholar]
  4. Franklin F. C. H., Bagdasarian M., Bagdasarian M. M., Timmis K. N. 1981; Molecular and functional analysis of the TOL plasmid pWW0 from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway.. Proceedings of the National Academy of Sciences of the United States of America 78:7458–7462
    [Google Scholar]
  5. Franklin F. C. H., Lehrbach P. R., Lurz R., Ruckert B., Bagdasarian M., Timmis K. N. 1983; Localisation and functional analysis of transposon mutations in regulatory genes of the TOL catabolic pathway.. Journal of Bacteriology 154:676–685
    [Google Scholar]
  6. Girvitz S. C., Bacchetti S., Rainbow A. J., Graham F. W. 1980; A rapid and efficient procedure for the purification of DNA from agarose gels.. Analytical Biochemistry 106:492–496
    [Google Scholar]
  7. Guerry P., Leblanc D. J., Falkow S. 1973; General method for isolation of plasmid deoxyribonucleic acid.. Journal of Bacteriology 116:1064–1066
    [Google Scholar]
  8. Harayama S., Lehrbach P. R., Timmis K. N. 1984; Transposon mutagenesis of meta-cleavage pathway operon genes of the TOL plasmid of Pseudomonas putida mt-2.. Journal of Bacteriology 160:251–255
    [Google Scholar]
  9. Holmes D. S., Quigley M. 1981; A rapid boiling method for preparation of bacterial plasmids.. Analytical Biochemistry 114:193–197
    [Google Scholar]
  10. Inouye S., Nakazawa A., Nakazawa T. 1981a; Molecular cloning of TOL genes xylB and xylE in Escherichia coli . Journal of Bacteriology 145:1143–1157
    [Google Scholar]
  11. Inouye S., Nakazawa A., Nakazawa T. 1981b; Molecular cloning of gene xylS of the TOL plasmid: evidence for the positive regulation of xylDEGF operon by xylS . Journal of Bacteriology 148:413–418
    [Google Scholar]
  12. Inouye S., Nakazawa A., Nakazawa T. 1983; Molecular cloning of regulatory gene xylR and operator promoter regions of the xylABC and xylDEGF operons of the TOL plasmid.. Journal of Bacteriology 155:1191–1199
    [Google Scholar]
  13. Inouye S., Ebina Y., Nakazawa A., Nakazawa T. 1984; Nucleotide sequence surrounding transcription initiation site of the xylABC operon on TOL plasmid of Pseudomonas putida . Proceedings of the National Academy of Sciences of the United States of America 81:1688–1691
    [Google Scholar]
  14. Jeenes D. J. 1982 The structure and properties of the TOL plasmid pWW0 in a chlorobenzoate-degrading Pseudomonas PhD thesis University College of N. Wales;
    [Google Scholar]
  15. Jeenes D. J., Williams P. A. 1982; Excision and integration of degradative pathway genes from TOL plasmid pWW0.. Journal of Bacteriology 150:188–191
    [Google Scholar]
  16. Jeenes D. J., Reineke W., Knackmuss H. -J., Williams P. A. 1982; TOL plasmid pWW0 in constructed halobenzoate-degrading Pseudomonas strains: enzyme regulations and DNA structure.. Journal of Bacteriology 150:180–187
    [Google Scholar]
  17. Lehrbach P. R., Jeenes D. J., Broda P. 1983; Characterisation by molecular cloning of insertion mutants in TOL catabolic functions.. Plasmid 9:112–125
    [Google Scholar]
  18. Maniatis T., Fritsch E. F., Sambrook J. (editors) 1982 Molecular Cloning: a Laboratory Manual pp 98–148 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  19. Mermod N., Lehrbach P. R., Reineke W., Timmis K. N. 1984; Transcription of the TOL plasmid toluate catabolic pathway operon of Pseudomonas putida is determined by a pair of co-ordinating and positively regulated overlapping promoters.. EMBO Journal 3:112461–2466
    [Google Scholar]
  20. Nakazawa T., Inouye S., Nakazawa A. 1980; Physical and functional mapping of RP4-TOL plasmid recombinants: analysis of insertion and deletion mutants.. Journal of Bacteriology 144:222–231
    [Google Scholar]
  21. Sala-Trepat J. M., Evans W. C. 1971; The meta cleavage of catechol by Azotobacter species: 4-oxocrotonate pathway.. European Journal of Biochemistry 20:400–413
    [Google Scholar]
  22. Timmis K. N., Lehrbach P. R., Harayama S., Den R. H., Mermod N., Bas S., Leppik R., Weightman A. J., Reineke W., Knackmuss H. -J. 1985; Analysis and manipulation of plasmid encoded pathways for the catabolism of aromatic compounds by soil bacteria.. In Plasmids in Bacteria pp 719–739 Edited by Habinski D. R., Cohen S. N., Clewell D. B., Jackson D. A., Hollaender A. New York: Plenum Press;
    [Google Scholar]
  23. Wheatcroft R., Williams P. A. 1981; Rapid methods for the study of both stable and unstable plasmids in Pseudomonas . Journal of General Microbiology 124:433–437
    [Google Scholar]
  24. Williams P. A., Murray K. 1974; Metabolism of benzoate and the methylbenzoates by Pseudomonas putida (arvilla) mt-2: evidence for the existence of a TOL plasmid.. Journal of Bacteriology 120:416–423
    [Google Scholar]
  25. Worsey M. J., Williams P. A. 1975; Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) mt-2: evidence for a new function of the TOL plasmid.. Journal of Bacteriology 124:7–13
    [Google Scholar]
  26. Worsey M. J., Franklin F. C. H., Williams P. A. 1978; Regulation of the degradative pathway enzymes coded by the TOL plasmid (pWW0) from Pseudomonas putida mt-2.. Journal of Bacteriology 134:757–764
    [Google Scholar]
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