1887

Abstract

Summary: Octyl β-D-glucopyranoside prevented the expression of cell-associated fructosyltransferase activity in ATCC 25975 grown in batch culture or incubated in non-proliferating cell suspension medium. This effect was not due to the direct inhibition of enzyme activity nor due to the loss of active enzyme into the external medium. The prevention of enzyme expression did not appear to be due to the inhibition of a general translocation mechanism for protein secretion, since fructosyltransferase activity was not detected within the cytoplasm of lysed cells grown in the presence of octyl β-D-glucopyranoside; nor was there any observed inhibition of the secretion of the extracellular enzyme glucosyltransferase. These and other observations supported the view that fructosyltransferase was not secreted across the cytoplasmic membrane in an active form before becoming associated with the cell surface.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-12-3243
1985-12-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/12/mic-131-12-3243.html?itemId=/content/journal/micro/10.1099/00221287-131-12-3243&mimeType=html&fmt=ahah

References

  1. Alonso A., Saez R., Villena A., Goni F. M. 1982; Increase in size of sonicated phospholipid vesicles in the presence of detergents.. Journal of Membrane Biology 67:55–62
    [Google Scholar]
  2. Chang C. N., Nielsen J. B. K., Isui K., Blobel G., Lampen J. O. 1982; Identification of the signal peptidase cleavage site in Bacillus licheniformis prepenicillinase.. Journal of Biological Chemistry 257:4340–4344
    [Google Scholar]
  3. Garszczynski S. M., Edwards J. R. 1973; Synthesis of a broth levan by a cell-bound levansucrase from Streptococcus salivarius . Archives of Oral Biology 18:239–251
    [Google Scholar]
  4. Helenius A., McCaslin D. R., Fries E., Tanford C. 1979; Properties of detergents.. Methods in Enzymology 56:734–750
    [Google Scholar]
  5. Ichihara S., Hussain M., Mizushima S. 1982; Mechanism of export of outer membrane lipoproteins through the cytoplasmic membrane in Escherichia coli . Journal of Biological Chemistry 257:495–500
    [Google Scholar]
  6. Jacques N. A. 1983; Membrane perturbation by cerulenin modulates glucosyltransferase secretion and acetate uptake by Streptococcus salivarius . Journal of General Microbiology 129:3293–3302
    [Google Scholar]
  7. Jacques N. A. 1984; Calcium dependence of the cell-associated fructosyltransferase of Streptococcus salivarius . Carbohydrate Research 127:349–355
    [Google Scholar]
  8. Jacques N. A., Wittenberger C. L. 1981; Inactivation of cell-associated fructosyltransferase in Streptococcus salivarius . Journal of Bacteriology 148:912–918
    [Google Scholar]
  9. Jacques N. A., Jacques V. L., Wolf A. C., Wittenberger C. L. 1985; Does an increase in membrane unsaturated fatty acids account for Tween 80 stimulation of glucosyltransferase secretion by Streptococcus salivarius?. Journal of General Microbiology 131:67–72
    [Google Scholar]
  10. Keevil C. W., West A. A., Bourne N., Marsh P. D. 1984; Inhibition of the synthesis and secretion of extracellular glucosyl- and fructosyltransferase in Streptococcus sanguis by sodium ions.. Journal of General Microbiology 130:77–82
    [Google Scholar]
  11. Knox K. W., Jacques N. A., Campbell L. K., Wicken A. J., Hurst S. F., Bleiweis A. S. 1979; Phenotypic stability of the cell wall of Streptococcus mutans Ingbritt grown under various conditions.. Infection and Immunity 26:1071–1078
    [Google Scholar]
  12. Markevics L. J., Jacques N. A. 1985; Enhanced secretion of glucosyltransferase by changes in potassium ion concentrations is accompanied by an altered pattern of membrane fatty acids in Streptococcus salivarius . Journal of Bacteriology 161:989–994
    [Google Scholar]
  13. Neilsen J. B. K., Lampen J. O. 1982; Membrane-bound penicillinases in Gram-positive bacteria.. Journal of Biological Chemistry 257:4490–4495
    [Google Scholar]
  14. Petit-Glatron M. F., Chambert R. 1981; Levansucrase of Bacillus subtilis: conclusive evidence that its production and export are unrelated to fatty acid synthesis but modulated by membrane-modifying agents.. European Journal of Biochemistry 119:603–611
    [Google Scholar]
  15. Russell R. R. B., Donald A. C., Douglas C. W. I. 1983a; Fructosyltransferase activity of a glucan-binding protein from Streptococcus mutans . Journal of General Microbiology 129:3243–3250
    [Google Scholar]
  16. Russell R. R. B., Peach S. L., Colman G., Cohen B. 1983b; Antibody responses to antigens of Streptococcus mutans in monkeys (Macaca fascicularis) immunized against dental caries.. Journal of General Microbiology 129:865–875
    [Google Scholar]
  17. Shockman G. D., Barrett J. F. 1983; Structure, function, and assembly of cell walls of Gram-positive bacteria.. Annual Review of Microbiology 37:501–527
    [Google Scholar]
  18. Siegel J. L., Hurst S. F., Lieberman E. S., Colman S. E., Bleiweis A. S. 1981; Mutanolysin-induced spheroplasts of Streptococcus mutans are true protoplasts.. Infection and Immunity 31:808–815
    [Google Scholar]
  19. Umesaki Y., Kawai Y., Mutai M. 1971; Effect of Tween 80 on glucosyltransferase production in Streptococcus mutans . Applied and Environmental Microbiology 34:115–119
    [Google Scholar]
  20. Ved H. S., Gustow E., Mahadevan V., Pieringer R. A. 1984; Dodecylglycerol. A new type of antibacterial agent which stimulates autolysin activity in Streptococcus faecium ATCC 9790.. Journal of Biological Chemistry 259:8115–8121
    [Google Scholar]
  21. West A. A., Keevil C. W., Marsh P. D., Ellwood D. C. 1984; The effect of ionophores on growth and glycosyltransferase production of Streptococcus sanguis . FEMS Microbiology Letters 25:133–137
    [Google Scholar]
  22. Wittenberger C. L., Beaman A. J., Lee L. N. 1978; Tween 80 effect on glucosyltransferase synthesis of Streptococcus salivarius . Journal of Bacteriology 133:231–239
    [Google Scholar]
  23. Wolfe P. B., Wickner W. 1984; Bacterial leader peptidase, a membrane protein without a leader peptide, uses the same export pathway as presecretory proteins.. Cell 36:1067–1072
    [Google Scholar]
  24. Yokogawa K., Kawata S., Takemura T., Yoshimura Y. 1975; Purification and properties of lytic enzymes from Streptomyces globisporus 1829.. Agricultural and Biological Chemistry 39:1533–1543
    [Google Scholar]
  25. Yu F., Furukawa H., Nakamura K., Mizushima S. 1984; Mechanism of location of major outer membrane lipoprotein in Escherichia coli. Studies with the omp F-lipoprotein hybrid protein.. Journal of Biological Chemistry 259:6013–6018
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-12-3243
Loading
/content/journal/micro/10.1099/00221287-131-12-3243
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error