1887

Abstract

Summary: The effects of elevated temperature and of digestion with a variety of proteinases on the floc-forming ability of flocculent strains of , both genetically defined and laboratory and genetically undefined brewing strains, have been determined. This has permitted classification of the flocculent phenotypes of these strains according to criteria other than quantitative grading of flocculence. The flocculent phenotypes conferred by both the and the gene were irreversibly lost upon treatment with pronase, proteinase K, trypsin or 2-mercaptoethanol treatments. However, the floc-forming ability of cells of the strain ABXL-1D was destroyed by chymotrypsin digestion and was stable to incubation at 70°C, whereas the floc-forming ability of cells of the strain ABXR-11A was resistant to the action of chymotrypsin and was heat labile. Tetrad analysis of a cross of these and strains indicated that the chymotrypsin and heat sensitivity phenotypes were -gene determined. It appears that expression of the and FLO5 genes leads to the production of different and characteristic cell-wall proteins underlying their respective flocculent phenotypes.

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/content/journal/micro/10.1099/00221287-131-12-3219
1985-12-01
2024-04-25
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References

  1. Amri M. A., Bonally R., Duteurtre B., Moll M. 1982; Yeast flocculation: influence of nutritional composition on cell wall composition.. Journal of General Microbiology 128:2001–2009
    [Google Scholar]
  2. Beavan M. J., Belk D. M., Stewart G. G., Rose A. H. 1979; Changes in electrophoretic mobility and lytic enzyme activity associated with development of flocculating ability in Saccharomyces cerevisiae . Canadian Journal of Microbiology 23:68–74
    [Google Scholar]
  3. Cryer D. R., Eccleshall R., Marmur J. 1975; Isolation of yeast DNA.. Methods in Cell Biology 12:39–44
    [Google Scholar]
  4. Holmberg S. 1978; Isolation and characterisation of a polypeptide absent from non-flocculent mutants of Saccharomyces cerevisiae . Carlsberg Research Communications 43:401–413
    [Google Scholar]
  5. Jayatissa P. M., Rose A. H. 1976; Role of wall phosphomannan in flocculation of Saccharomyces cerevisiae . Journal of General Microbiology 96:165–174
    [Google Scholar]
  6. Johnston J. R., Lewis C. W. 1974; Genetic analysis of flocculation in Saccharomyces cerevisiae and tetrad analysis of commercial brewing and baking yeasts.. In Second International Symposium on the Genetics of Industrial Microorganisms pp 339–355 Edited by MacDonald K. D. London: Academic Press;
    [Google Scholar]
  7. Johnston J. R., Mortimer R. K. 1984; Pedigree of the Yeast Genetic Stock Center ‘wild-type’ strains S288C and X2180 of S. cerevisiae . Heredity 52:459
    [Google Scholar]
  8. Johnston J. R., Reader H. P. 1983; Genetic control of flocculation.. In Yeast Genetics: Fundamental and Applied Aspects pp 205–224 Edited by Spencer J. F. T., Spencer D. M., Smith A. R. W. New York: Springer-Verlag;
    [Google Scholar]
  9. Lewis C. W. 1974 Genetic and related aspects of flocculation in yeast PhD thesis University of Strathclyde, Glasgow;
    [Google Scholar]
  10. Lewis C. W., Johnston J. R., Martin P. A. 1976; The genetics of yeast flocculation.. Journal of the Institute of Brewing 82:158–160
    [Google Scholar]
  11. Lyons T. P., Hough J. S. 1971; Further evidence for the cross-bridging hypothesis for flocculation of brewer's yeast.. Journal of the Institute of Brewing 77:300–305
    [Google Scholar]
  12. Mill P. J. 1964; The nature of the interactions between flocculent cells in the flocculation of Saccharomyces cerevisiae . Journal of General Microbiology 35:61–68
    [Google Scholar]
  13. Miki B. L. A., Poon N. H., James A. P., Seligy V. L. 1980; Flocculation in Saccharomyces cerevisiae: mechanism of cell–cell interaction.. In Current Developments in Yeast Research pp 165–170 Edited by Stewart G., Russell I. Toronto: Pergamon Press, Canada;
    [Google Scholar]
  14. Miki B. L. A., Poon N. H., Seligy V. L. 1982; Repression and induction of flocculation interactions in Saccharomyces cerevisiae . Journal of Bacteriology 150:890–899
    [Google Scholar]
  15. Nishihara H., Toraya T., Fukui S. 1977; Effect of chemical modification of cell surface components of a brewer's yeast on the floc-forming ability.. Archives of Microbiology 115:19–23
    [Google Scholar]
  16. Nishihara H., Toraya T., Fukui S. 1982; Flocculation of cell walls of brewer's yeast and effects of metal ions, protein-denaturants and enzyme treatments.. Archives of Microbiology 131:112–115
    [Google Scholar]
  17. Russell I., Stewart G. G., Reader H. P., Johnston J. R., Martin P. A. 1980; Revised nomenclature of genes that control yeast flocculation.. Journal of the Institute of Brewing 86:120–121
    [Google Scholar]
  18. Sherman F., Fink G. R., Hicks J. B. 1983 Methods in Yeast Genetics Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  19. Stewart G. G., Russell I. 1977; The identification, characterization and mapping of a gene for flocculation in Saccharomyces sp.. Canadian Journal of Microbiology 23:441–447
    [Google Scholar]
  20. Stewart G. G., Russell I. 1981; Yeast flocculation.. In Brewing Science vol 2 pp 61–92 Edited by Pollock J. R. A. London: Academic Press;
    [Google Scholar]
  21. Yamashita I., Fukui S. 1983; Mating signals control expression of both starch fermentation genes and a novel flocculation gene FLO8 in the yeast Saccharomyces . Agricultural and Biological Chemistry 47:2889–2896
    [Google Scholar]
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