1887

Abstract

Summary: A degradation pathway for the acetylenic compound 3-butyn-1-ol by a bacterium showing strong resemblance to the physiologically well characterized AM1 is presented. Enzyme studies revealed that the pathway of 3-butyn-1-ol metabolism involves an initial oxidation of the alcohol group by a phenazine methosulphate dependent alcohol dehydrogenase. Subsequently, the aldehyde is probably oxidized to yield 3-butynoic acid. The latter product is hydrated in the absence of cofactors to give acetoacetate which, in turn, is further degraded via acetoacetyl-CoA to acetyl-CoA. The inducible hydration reaction was catalysed by a nonspecific enzyme; hydration of propynoic acid resulted in the formation of malonic semialdehyde.

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/content/journal/micro/10.1099/00221287-131-12-3155
1985-12-01
2021-05-09
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References

  1. Anthony C., Zatman L. J. 1965; The microbial oxidation of methanol. The alcohol dehydrogenase of Pseudomonas sp. M27.. Biochemical Journal 96:808–812
    [Google Scholar]
  2. Boulton C. A., Large P. J. 1977; Synthesis of certain assimilatory and dissimilatory enzymes during bacterial adaptation to growth on trimethylamine.. Journal of General Microbiology 101:151–156
    [Google Scholar]
  3. Colby J., Zatman L. J. 1973; Trimethylamine metabolism in obligate and facultative methylotrophs.. Biochemical Journal 132:101–112
    [Google Scholar]
  4. De Bont J. A. M., Peck M. W. 1980; Metabolism of acetylene by Rhodococcus. A1.. Archives of Microbiology 127:99–104
    [Google Scholar]
  5. De Bont J. A. M., Scholten J., van den Tweel W. J. J. 1985; Isolation of microorganisms on 3-butyn-1-ol and other acetylenic compounds.. Current Microbiology (in the Press)
    [Google Scholar]
  6. Dijkstra M., van den Tweel W. J. J., De Bont J. A. M., Frank J., Jzn & Duine J. A. 1985; Monomeric and dimeric quinoprotein alcohol dehydrogenase from alcohol-grown Pseudomonas BB1.. Journal of General Microbiology 131:3163–3169
    [Google Scholar]
  7. Groeneveld A., Dijkstra M., Duine J. A. 1984; Cyclopropanol in the exploration of bacterial alcohol oxidation.. FEMS Microbiology Letters 25:311–314
    [Google Scholar]
  8. Hayaishi O., Nishizuka Y., Tatibana M., Takashita M., Kuno S. 1961; Enzymatic studies on the metabolism of β-alanine.. Journal of Biological Chemistry 236:781–790
    [Google Scholar]
  9. Heilbron I., Jones E. R. H., Sondheimer F. 1949; Researches on acetylenic compounds. XV. The oxidation of primary acetylenic carbinols and glycols.. Journal of the Chemical Society604–607
    [Google Scholar]
  10. Johnson P. A., Quayle J. R. 1982; Microbial growth on C1 compounds. Oxidation of methanol, formaldehyde and formate by methanol-grown Pseudomonas AM1.. Biochemical Journal 93:281–290
    [Google Scholar]
  11. Kanner D., Bartha R. 1982; Metabolism of acetylene by Nocardia rhodochrous . Journal of Bacteriology 150:989–992
    [Google Scholar]
  12. Krebs H. A., Eggleston L. V. 1945; Metabolism of acetoacetate in animal tissues. I.. Biochemical Journal 39:408–419
    [Google Scholar]
  13. Marison I. W., Attwood M. M. 1980; Partial purification and characterization of a dye-linked formaldehyde dehydrogenase from Hyphomicrobium X.. Journal of General Microbiology 117:305–313
    [Google Scholar]
  14. Miyoshi T., Sato H., Harada T. 1974; Purification and characterization of 2-alkyne-1-ol dehydrogenase induced by 2-butyne-1,4-diol in Fusarium merismoides B11.. Biochimica et biophysica acta 358:231–239
    [Google Scholar]
  15. Peel D., Quayle J. R. 1961; Microbial growth on C1 compounds. Isolation and characterization of Pseudomonas AM1.. Biochemical Journal 81:465–469
    [Google Scholar]
  16. Senior P. J., Dawes E. A. 1973; The regulation of poly-β-hydroxybutyrate metabolism in Azotobacter beijerinckii . Biochemical Journal 134:225–238
    [Google Scholar]
  17. Stirling D. I., Dalton H. 1978; Purification and properties of a NAD(P)+-linked formaldehyde dehydrogenase from Methylococcus capsulatus (Bath).. Journal of General Microbiology 107:19–29
    [Google Scholar]
  18. Taylor I. J., Anthony C. 1976; Acetyl-CoA production and utilization during growth of the facultative methylotroph Pseudomonas AM1 on ethanol, malonate and 3-hydroxybutyrate.. Journal of General Microbiology 95:134–143
    [Google Scholar]
  19. Walker P. G. 1954; A colorimetric method for the estimation of acetoacetate.. Biochemical Journal 58:699–704
    [Google Scholar]
  20. Yamada E. W., Jakoby W. B. 1958; Enzymatic utilization of acetylenic compounds. An enzyme converting acetylenedicarboxylic acid to pyruvate.. Journal of Biological Chemistry 233:706–711
    [Google Scholar]
  21. Yamada E. W., Jakoby W. B. 1959; Enzymatic utilization of acetylenic compounds. Acetylene-monocarboxylic acid hydrase.. Journal of Biological Chemistry 234:941–945
    [Google Scholar]
  22. Yamanaka K. 1981; Comparative aspects of methanol dehydrogenases.. In Proceedings of the Third International Symposium on Microbial Growth on C1-compounds pp 21–30 Edited by Dalton H. London: Heyden & Son;
    [Google Scholar]
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