RT Journal Article SR Electronic(1) A1 Ichimaru Ogawa, Hiroko A1 Nakamura, M.YR 1985 T1 Characterization of a Mycoplasma Virus (MV-O1) Derived from and Infecting Acholeplasma oculi JF Microbiology, VO 131 IS 11 SP 3117 OP 3126 DO https://doi.org/10.1099/00221287-131-11-3117 PB Microbiology Society, SN 1465-2080, AB Summary: A new Mycoplasmatales virus, referred to as MV-O1, was isolated during cloning of Acholeplasma oculi 19L. The virus formed plaques only on strains of A. oculi, i.e. the original clone, A. oculi 19L, a subclone of A. oculi 19L (A. oculi-i), A. oculi Goat 5 and a wild isolate (K-2) of A. oculi, but not on other acholeplasmas, including strains of A. laidlawii, nor on five human mycoplasma species tested. The virus required horse serum for multiplication as well as for plaque formation and passed through a 100 nm filter. Electron microscopy revealed enveloped, spherical particles 80-130 nm in diameter. The buoyant density of purified virus was 1.23 g ml−1 in CsCl, and agarose gel electrophoresis indicated that the viral nucleic acid was DNA., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-131-11-3117