1887

Abstract

Summary: The electrophoretic mobilities of 12 enzymes from 19 species (including 6 strains of and were characterized by polyacrylamide slab gel electrophoresis. All strains and species tested exhibited qualitatively different zymogram patterns. Species and strain relationships were quantified by pairwise comparisons of all 12 enzyme systems to obtain similarity indices; these data were subjected to numerical clustering methods to obtain groups and a phenogram. The electrophoretic classification compared favourably with those obtained by other criteria. In addition, the quantitative clustering data indicated that and are sufficiently different from the other species to warrant their separation into a distinct group. These two species also lacked the characteristic NADPH-diaphorase zymogram pattern found in all the other species. Intra-species similarity indices were generally greater than the inter-species index values. However, certain species such as and had similarity index values in the range of inter-strain index values.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-11-3105
1985-11-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/11/mic-131-11-3105.html?itemId=/content/journal/micro/10.1099/00221287-131-11-3105&mimeType=html&fmt=ahah

References

  1. Avise J. C. 1974; Systematic value of electrophoretic data.. Systematic Zoology 23:465–481
    [Google Scholar]
  2. Avise J. C., Aquadro C. F. 1982; A comparative summary of genetic distances in the vertebrates: patterns and correlations.. In Evolutionary Biology vol 15 pp. 151–185 Edited by Hecht M. K., Wallace B., Prance G. T. New York: Plenum Press;
    [Google Scholar]
  3. Ayala F. J. editor 1978 Molecular Evolution Sutherland Massachusetts: Sinauer Associates;
    [Google Scholar]
  4. Baptist J. N., Shaw C. R., Mandel M. 1969; Zone electrophoresis of enzymes in bacterial taxonomy.. Journal of Bacteriology 99:180–188
    [Google Scholar]
  5. Berger U. 1960; Neisseria haemolysans (Thjotta and Boe, 1938): Untersuchungen zur Stellung im System.. Zeitschrift fur Hygiene und Infektionskrankheiten 146:253–259
    [Google Scholar]
  6. B∅vre K. 1980; Progress in classification and identification of Neisseriaceae based on genetic affinity.. In Microbiological Classification and Identification pp. 55–72 Edited by Goodfellow M., Board R. G. New York: Academic Press;
    [Google Scholar]
  7. Chun P. K., Gordon A. M., Vedros N. A., Sensabaugh G. F. 1984; Strain differentiation of pathogenic Neisseria strains by enzyme electrophoresis.. In Abstracts of the Annual Meeting of the American Society for Microbiology abstract no. C-263 Washington, DC: American Society for Microbiology;
    [Google Scholar]
  8. Giard R., Vedros N. A. 1981; Biphasic resin system for growing concentrated cultures of microorganisms and its application to the cultivation of Neisseria gonorrhoeae. Applied and Environmental Microbiology 41:846–847
    [Google Scholar]
  9. Harris H., Hopkinson D. A. 1976 Handbook of Enzyme Electrophoresis in Human Genetics New York: American Elsevier;
    [Google Scholar]
  10. Hoke C., Vedros N. A. 1982a; Taxonomy of the Neisseriae: fatty acid analysis, aminopeptidase activity, and pigment extraction.. International Journal of Systematic Bacteriology 32:51–56
    [Google Scholar]
  11. Hoke C., Vedros N. A. 1982 b; Taxonomy of the Neisseriae: deoxyribonucleic acid base composition, interspecific transformation, and deoxyribonucleic acid hybridization.. International Journal of Systematic Bacteriology 32:57–66
    [Google Scholar]
  12. Holten E. 1974a; Glucokinase and glucose-6-phosphate dehydrogenase InNeisseria. Acta pathologica et microbiologica scandinavica B82:201–206
    [Google Scholar]
  13. Holten E. 1974 b; Immunological comparison of NADP-dependent glutamate dehydrogenase and malate dehydrogenase in genus Neisseria. Acta pathologica et microbiologica scandinavica B82:849–859
    [Google Scholar]
  14. Kingsbury D. T. 1967; Deoxyribounucleic acid homologies among species of the genus Neisseria. Journal of Bacteriology 94:870–874
    [Google Scholar]
  15. Knapp J. S., Totten P. A., Minshew B. H., Hook E. W. 1983; Neisseria cinerea: a nonpathogenic Neisseria sp. related to Neisseria gonorrhoeae. In Abstracts of the Annual Meeting of the American Society for Microbiology p. 316 Washington, DC: American Society for Microbiology;
    [Google Scholar]
  16. Nei M. 1972; Genetic distance between populations.. American Naturalist 106:283–292
    [Google Scholar]
  17. Nei M. 1975 Molecular Population Genetics and Evolution Amsterdam: North Holland;
    [Google Scholar]
  18. Ornstein L. 1964; Disc electrophoresis. I. Background and theory.. Annals of the New York Academy of Sciences 121:321–349
    [Google Scholar]
  19. Reyn A. 1974; The genus Neisseria. In Bergey’s. Manual of Determinative Bacteriology, 8th. edn pp. 428–432 Edited by Buchanan R. E., Gibbons N. E. Baltimore: Williams Wilkins;
    [Google Scholar]
  20. Shaw C. R., Prasad J. 1970; Starch gel electrophoresis of enzymes: a compilation of recipes.. Biochemical Genetics 4:297–320
    [Google Scholar]
  21. Sneath P. H. A., Sokal R. 1973 Numerical Taxonomy: the Principles and Practices of Numerical Classification San Francisco: W. H. Freeman;
    [Google Scholar]
  22. Vedros N. A. 1981; The genus Neisseria. In The Prokaryotes: a Handbook on Habitats, Isolation, and Identification of Bacteria vol. 2 pp, 1499–1505 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. New York: Springer-Verlag;
    [Google Scholar]
  23. Vedros N. A., Hoke C., Chun P. K. 1983; Neisseria macacae sp. nov., a new Neisseria species isolated from the oropharynx of Rhesus Monkeys (Macaca mulatto).. International Journal of Systematic Bacteriology 33:515–520
    [Google Scholar]
  24. Wilson A. C., Carlson S. C., White T. J. 1977; Biochemical evolution.. Annual Review of Biochemistry 46:573–639
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-11-3105
Loading
/content/journal/micro/10.1099/00221287-131-11-3105
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error