1887

Abstract

Summary: Various micro-organisms (131 strains of 73 species) were studied for their ability to produce thymidine kinase (TK; EC 2.7.1.21). Taking the specific TK activity of K12 [specific activity of sonicated cell extracts 95–194 pmol min (mg protein)] as 100%, the test organisms had the following relative specific TK activities. In the Gram-positive cocci, (21–84%) showed higher activity than (1–20%) and (1–7%) except for one strain of (29%). , a Gram-negative coccus, lacked TK. Gram-positive endospore-forming rods showed significant activity (, 13–51%; . 9–18%) except for one strain of (2%) and (1–3%). Among the Gram-positive asporogenous rods, and six species of (especially and ) had moderate to high activity (23–348%) but , and had low activity (0–8%). Of the species of studied, most lacked TK but and had significant TK activity (15–53%). Of the Gram-negative facultative anaerobes, lacked TK, while , including (148–1120%), (59–141%), (78–299%) and (61–110%), had a high activity. had a somewhat lower activity (0–34%) except for ‘’ (307%). and and related organisms other than and lacked TK. The seven species of tested, and , essentially lacked TK. The distribution of TK among various bacteria showed some correlation with a phylogenic tree based on 5S rRNA sequences; in particular, organisms with 5S rRNA of the 118N-type did not produce TK.

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1985-11-01
2024-03-29
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