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Summary:pDC250 is a 52 kb conjugative cryptic plasmid from Erwinia stewartii strain SW2. This plasmid and its transposon-carrying derivatives, pDC251 and pDC252, were transmissible to E. chrysanthemi, E. amylovora, E. milletiae, E. herbicola, Escherichia coli, Shigella flexneri and Salmonella arizonae. In Esch. coli, E.stewartii and E. chrysanthemi, pDC251 was stable and derepressed for conjugal transfer. Hybridization studies confirmed thatpDC250 is widely distributed among E.stewartii strains and showed that it was also closely related to 34 kb plasmids found in strains lacking a 52kb plasmid. The 34 kb plasmid from E.stewartii strain SSI04, pDC140, was transformed into Esch. coli and compared with pDC250. Restriction mapping of the twoplasmids revealed a nearly identical 20 kb region. In Esch.coli, pDC140 was unstable in the presence of pDC250.
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