Summary: pDC250 is a 52 kb conjugative cryptic plasmid from strain SW2. This plasmid and its transposon-carrying derivatives, pDC251 and pDC252, were transmissible to and . In and , pDC251 was stable and derepressed for conjugal transfer. Hybridization studies confirmed that pDC250 is widely distributed among strains and showed that it was also closely related to 34 kb plasmids found in strains lacking a 52 kb plasmid. The 34 kb plasmid from strain SS104, pDC140, was transformed into and compared with pDC250. Restriction mapping of the two plasmids revealed a nearly identical 20 kb region. In , pDC140 was unstable in the presence of pDC250.


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