1887

Abstract

Summary:pDC250 is a 52 kb conjugative cryptic plasmid from strain SW2. This plasmid and its transposon-carrying derivatives, pDC251 and pDC252, were transmissible to and In and , pDC251 was stable and derepressed for conjugal transfer. Hybridization studies confirmed thatpDC250 is widely distributed among strains and showed that it was also closely related to 34 kb plasmids found in strains lacking a 52kb plasmid. The 34 kb plasmid from strain SSI04, pDC140, was transformed into and compared with pDC250. Restriction mapping of the twoplasmids revealed a nearly identical 20 kb region. In , pDC140 was unstable in the presence of pDC250.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-11-2985
1985-11-01
2021-05-07
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/11/mic-131-11-2985.html?itemId=/content/journal/micro/10.1099/00221287-131-11-2985&mimeType=html&fmt=ahah

References

  1. Ashbaugh M. F. 1980; Conjugal transfer of drug resistance plasmids of different incompatibility groups into Erwinia stewartii MSc thesis. The Ohio State University; USA:
    [Google Scholar]
  2. Bachmann B. J. 1972; Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriological Reviews 36:525–557
    [Google Scholar]
  3. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  4. Bolivar F., Backman K. 1979; Plasmids of Escherichia coli as cloning vectors. Methods in Enzymology 68:245–267
    [Google Scholar]
  5. Chatterjee A. K., Starr M. P. 1973; Transmission of lac by the sex factor E in Erwinia strains from human clinical sources. Infection and Immunity 8:563–572
    [Google Scholar]
  6. Coplin D. L. 1978; Properties of F and P group plasmids in Erwinia stewartii. Phytopathology 68:1637–1643
    [Google Scholar]
  7. Coplin D. L., Rowan R. G. 1978; Conjugative plasmids in Erwinia stewartii. In Proceedings of the 4th International Conference on Plant Pathogenic Bacteria pp 67–73 Angers, France: Station de Pathologie Végétaleet Phytobactériologie, Institut National de la RechercheAgronomique;
    [Google Scholar]
  8. Coplin D. L., Rowan R. G., Chisholm D. A., Whitmoyer R. E. 1981; Characterization of plasmids in Erwinia stewartii. Applied and Environmental Microbiology 42:599–604
    [Google Scholar]
  9. Currier T. C., Morgan M. K. 1981; Restriction endonuclease analysis of the incompatibility group P-1 plasmids RK2, RP1, RP4, R68, and R68.45. Current Microbiology 5:323–327
    [Google Scholar]
  10. Currier T. C., Nester E. W. 1976; Isolation of covalently closed circular DNA of high molecular weight from bacteria. Analytical Biochemistry 76:431–441
    [Google Scholar]
  11. Gantotti B. V., Beer S. V. 1982; Plasmid-borne determinants of pigmentation and thiamine prototrophy in Erwinia herbicola . Journal of Bacteriology 151:1627–1629
    [Google Scholar]
  12. Hershfield V., Boyer H. W., Yanofsky C., Lovett M. A., Helinski D. R. 1974; Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA. Proceedings of the National Academy of Sciences of the United States of America 71:3455–3459
    [Google Scholar]
  13. Kretschmer P. J., Chang A. C. Y., Cohen S. N. 1975; Indirect selection of bacterial plasmids lacking identifiable phenotypic properties. Journal of Bacteriology 124:225–231
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  15. Mccammon S. L., Coplin D. L., Rowan R. G. 1985; Isolation of avirulent mutants of Erwinia stewartii using bacteriophage Mu pf7701. Journal of General Microbiology 131:2993–3000
    [Google Scholar]
  16. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Parker R. C., Seed B. 1980; Restriction digests in the presence of agarose. Methods in Enzymology 65:358–363
    [Google Scholar]
  19. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by agarose gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  20. Thiry G. 1984; Plasmids of the epiphytic bacterium Erwinia uredovora. Journal of General Microbiology 130:1623–1631
    [Google Scholar]
  21. Thiry G., Faelen M. 1984; Erwinia uredovora 20D3 contains an IncM plasmid. Journal of General Microbiology 130:1633–1640
    [Google Scholar]
  22. Wahl G., Stern M., Stark G. R. 1979; Efficient transfer of large DNA fragments from agarose gels to diazobenzylomethoxymethyl paper and rapid hybridization using dextran sulfate. Proceedings of the National Academy of Sciences of the United States of America 76:3683–3687
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-11-2985
Loading
/content/journal/micro/10.1099/00221287-131-11-2985
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error