1887

Abstract

Summary: Pigment–protein complexes from were prepared by detergent solubilization of intra-cytoplasmic membranes followed by gel electrophoresis or sucrose gradient centrifugation. These procedures gave rise to two native pigmented complexes. The major one (designated B800-865) was associated with two polypeptides of 11000 and 13000 and was identified with the ‘accessory’ light-harvesting complex II found in other members of the The minor complex (designated B885-RC) contained both reaction centre and light-harvesting bacteriochlorophyll. Detergent fractionation and reversible chemical cross-linking, followed by two-dimensional polyacrylamide gel electrophoresis, indicated a specific relationship between a membrane-bound cytochrome -553 and the 31000 subunit of the reaction centre.

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1985-11-01
2021-05-06
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