1887

Abstract

Summary: Autolysis of purified cell walls resulted in the release of several components thought to be mannoprotein in nature, since they were retained by Concanavalin A-Sepharose. The most abundant was a 29 kDal molecule which was also a major species among mannoproteins solubilized by the -glucanase complex Zymolyase. There was a concomitant release of glucose and mannose oligosaccharides and of -glucanase activity. Glucanase and protease inhibitory treatments considerably lowered the release of mannoproteins. The use of different protease inhibitors during autolytic incubations interfered with at least two proteases (one of them being a cysteine protease) apparently involved in the solubilization and partial degradation of mannoproteins from the walls.

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1985-11-01
2024-04-25
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References

  1. Abd-El-Al A.T.H., Phaff H.J. 1968; Exo-β-glucanases in yeast. Biochemical Journal 109:347–360
    [Google Scholar]
  2. Bacon J. S. D., Farmer V. C., Jones D., Taylor I.F. 1969; The glucan components of the cell wall of baker’s yeast (Saccharomyces cerevisiae) considered in relation to its ultrastructure. Biochemical Journal 114:557–576
    [Google Scholar]
  3. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  4. Elango N., Correa J. U., Cabib E. 1982; Secretory character of yeast chitinase. Journal of Biological Chemistry 257:1398–1400
    [Google Scholar]
  5. Elorza M. V., Murgui A., Sentandreu R. 1985; Dimorphism in Candida albicans:contribution of mannoproteins to the architecture of yeast and mycelial cell walls. Journal of General Microbiology 131:2209–2216
    [Google Scholar]
  6. Farkas V., Biely P., Bauer S. 1973; Extracellular β-glucanases of the yeast Saccharomyces cerevisiae. Biochimica et biophysica acta 21:246–255
    [Google Scholar]
  7. Fleet G. H. 1984 The occurrence and function of endogenous wall-degrading enzymes in yeast. In Microbial Cell Wall Synthesis and Autolysis pp 227–238 Edited by Nombela C. Amsterdam: Elsevier;
    [Google Scholar]
  8. Fleet G. H., Manners D. J. 1977; The enzymic degradation of an alkali-soluble glucan from the cell walls of Saccharomyces cerevisiae. Journal of General Microbiology 98:315–327
    [Google Scholar]
  9. Fleet G. H., . Phaff H. J. 1974; Glucanases in Schizosaccharomyces. Isolation and properties of the cell wall associated (l→3)-β-glucanases. Journal of Biological Chemistry 249:1717–1728
    [Google Scholar]
  10. Frey J., Röhm K. H. 1979; External and internal forms of yeast aminopeptidase II. European Journal of Biochemistry 97:169–173
    [Google Scholar]
  11. Hien N. H., Fleet G. H. 1983a; Separation and characterization of six (1 →3)-β-glucanases from Saccharomyces cerevisiae. Journal of Bacteriology 156:1204–1213
    [Google Scholar]
  12. Hien N. H., Fleet G. H. 1983b; Variation of (1 →3)-β-glucanases in Saccharomyces cerevisiae during vegetative growth, conjugation and sporulation. Journal of Bacteriology 156:1214–1220
    [Google Scholar]
  13. Kitamura K., Kaneko T., Yamamoto Y. 1974; Lysis of viable yeast cells by enzymes of Arthrobacter luteus. II. Purification and properties of an enzyme, Zymolyase, which lyses viable yeast cells. Journal of General and Applied Microbiology 20:323–344
    [Google Scholar]
  14. Maddox I. S., Hough J. S. 1971; Yeast glucanase and mannanase. Journal of the Institute of Brewing 77:44–47
    [Google Scholar]
  15. Nelson N. 1944; A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry 153:375–380
    [Google Scholar]
  16. Notario V. 1982; β-Glucanases from Candida albicans: purification, characterization and the nature of their attachment to cell wall components. Journal of General Microbiology 128:747–759
    [Google Scholar]
  17. Notario V., Villa T. G., Villanueva J. R. 1976; Purification of an exo-β-D-glucanase from cell-free extracts of Candida utilis. Biochemical Journal 159:555–562
    [Google Scholar]
  18. Pastor F. I. J., Valentin E., Herrero E., Sentandreu R. 1984; Structure of the Saccharomyces cerevisiae cell wall: mannoproteins released by zymolyase and their contribution to wall architecture. Biochimica et biophysica acta 802:292–300
    [Google Scholar]
  19. Phaff H. J. 1971; Structure and biosynthesis of the yeast cell envelope. In The Yeasts Vol. 2 Physiology and Biochemistry of Yeasts pp 135–210 Edited by Rose A. H., Harrison J. S. London: Academic Press;
    [Google Scholar]
  20. Phaff H. J. 1977; Enzymatic yeast cell wall degradation. Advances in Chemistry 160:244–282
    [Google Scholar]
  21. Reichelt B. Y., Fleet G. H. 1981; Isolation, properties, function and regulation of endo-(l→3)-β-glucanases in Schizosaccharomyces pombe. Journal of Bacteriology 147:1085–1094
    [Google Scholar]
  22. Rey F. J., Santos T., Garcia-Acha I., Nombela A. C. 1979; Synthesis of 1→3-β-glucanases in Saccharomyces cerevisiae during the mitotic cycle, mating and sporulation. Journal of Bacteriology 139:924–931
    [Google Scholar]
  23. Russell L., Garrison I. F., Stewart G. G. 1973; Studies on the formation of sphaeroplasts from stationary phase cells of Saccharomyces cerevisiae. Journal of the Institute of Brewing 79:48–54
    [Google Scholar]
  24. Saheki T., Holzer H. 1974; Comparisons of the tryptophan synthase inactivating enzymes with proteinases from yeast. European Journal of Biochemistry 42:621–626
    [Google Scholar]
  25. Sanchez A., Villanueva J. R., Villa T. G. 1982; Saccharomyces cerevisiae secretes two exo-β-glucanases. FEBS Letters 138:209–212
    [Google Scholar]
  26. Sentandreu R., Herrero E., Martinez J. P., Larriba G. 1984; Biogenesis of the yeast cell wall. Subcellular Biochemistry 10:193–235
    [Google Scholar]
  27. Shibata N., Mizugami K., Takano K., Suzuki S. 1983; Isolation of mannan-protein complexes from viable cells of Saccharomyces cerevisiae X2180-1A wild type and Saccharomyces cerevisiae X2180-1A-5 mutant strains by the action of Zymolyase-60,000. Journal of Bacteriology 156:552–558
    [Google Scholar]
  28. Somogyi M. 1952; Notes on sugar determination. Journal of Biological Chemistry 195:19–23
    [Google Scholar]
  29. Trumbly R., Bradley G. 1983; Isolation and characterization of aminopeptidase mutants of Saccharomyces cerevisiae. Journal of Bacteriology 156:36–48
    [Google Scholar]
  30. Valentin E., Herrero E., Pastor F. I. J., Sentandreu R. 1984; Solubilization and analysis of mannoprotein molecules from the cell wall of Saccharomyces cerevisiae. Journal of General Microbiology 130:1419–1428
    [Google Scholar]
  31. Villa T. G., Lachance M. A., Phaff H. J. 1978; β-Glucanases of the yeast Kluyveromyces phaseolo-sporus: partial purification and characterization. Experimental Mycology 2:12–25
    [Google Scholar]
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