1887

Abstract

Summary: Two-dimensional diffusion gradients (of NaCl and H concentrations) were established in solid growth medium containing glycerol and yeast extract as major carbon sources. These were used to investigate conditions favourable for growth and bioluminescence of three species of luminous bacteria during incubation at different temperatures. and all grew over the entire salt range used [0.9–3% (w/v) NaCl] and at pH values < 7 at the most favourable temperatures (20 °C, 20 °C and 15 °C respectively); upper and lower temperature limits for growth over a 72 h period were 30 and 10, 25 and 5, and 30 and 5 °C respectively. Bioluminescence was observed at all temperatures that supported growth; in emission at 10 °C was hardly detectable even after 72 h, but at higher temperatures it occurred at all NaCl concentrations. Low pH values and high NaCl concentrations favoured luminescence in the other two organisms; after 48 h light emission decreased from the high pH and low NaCl regions of the gels. These results are discussed with reference to the symbiotic () or free-living () origins of the organisms studied.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-11-2865
1985-11-01
2021-05-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/11/mic-131-11-2865.html?itemId=/content/journal/micro/10.1099/00221287-131-11-2865&mimeType=html&fmt=ahah

References

  1. Dunlap P.V. 1984; Physiological and morphologi-cal state of the symbiotic bacteria from light organsof ponyfish.. Biological Bulletin 167:410–425
    [Google Scholar]
  2. Dunlap P.V. 1985; Osmotic control of luminescenceand growth in Photobacterium leiognathi from pony-fish light-organs.. Archives of Microbiology 141:44–50
    [Google Scholar]
  3. Harvey E.N. 1952; Bioluminescence.. New York: Academic Press;
    [Google Scholar]
  4. Hastings J.W., Nealson K.H. 1977; Bacterialbioluminescence.. Annual Review of Microbiology 31:549–595
    [Google Scholar]
  5. Hastings J.W., Nealson K.H. 1981; Thesymbiotic luminous bacteria.. In The Prokaryotes PP 1332–1345 Edited by Starrr M.p., Stolp H., Trüper H., Ballows A., Schlegel H.G. New York: Springer;
    [Google Scholar]
  6. Hastings J. W., Potrikus C. J., Guptha S. C., Kurfurst M., Makemson J. C. 1985; Biochem-istry and physiology of bioluminescent bacteria. Advances in Microbial Physiology. Biological Bulletin 26:235–291
    [Google Scholar]
  7. Makemson J. L., Hastings J. W. 1982; Iron represses bioluminescence and affects catabolite repression of luminescence in Vibrio harveyi.. Current Microbiology 7:181–186
    [Google Scholar]
  8. Nealson K. H., Hastings J. W. 1979; Bacterial bioluminescence: its control and ecological significance.. Microbiological Reviews 43:496–518
    [Google Scholar]
  9. O’Brien C. H., Sizemore R. K. 1979; Distribution of the luminous bacterium Beneckea harveyi in a semitropical estuarine environment.. Applied and Environmental Microbiology 38:928–933
    [Google Scholar]
  10. Prosser C. L. 1973; Comparative Physiology. 3rd edn. Philadelphia: Saunders College;
    [Google Scholar]
  11. Ruby E. G., Nealson K. H. 1976; Symbiotic association of Photobacterium fischeri with the marine luminous fish Monocentris japonica: a modelof symbiosis based on bacterial studies.. BiologicalBulletin 151:574–586
    [Google Scholar]
  12. Ruby E. G., Nealson K. H. 1978; Seasonalchanges in the species composition of luminousbacteria in nearshore seawater.. Limnology andOceanography 23:530–533
    [Google Scholar]
  13. Ruby E. G., Greenberg E. P., Hastings J. W. 1980; Planktonic marine luminous bacteria: spe-cies distribution on the water column.. Applied and Environmental Microbiology 39:302–306
    [Google Scholar]
  14. Shilo M., Yetinson T. 1979; Physiologicalcharacteristics underlying the distribution patternsof luminous bacteria in the Mediterranean Sea and the Gulf of Elat.. Applied and Environmental Microbiology 38:577–584
    [Google Scholar]
  15. Szybalski W. 1952; Gradient plates for the study of microbial resistance to antibiotics.. Bacteriological Proceedings 36:
    [Google Scholar]
  16. Szybalski W., Bryson V. 1952; Genetic studies on microbial cross resistance to toxic agents. I. Cross resistance of Escherichia coli to fifteen antibiotics.. Journal of Bacteriology 64:489–499
    [Google Scholar]
  17. Waters C. A., Hastings J. W. 1977; Mutants of luminous bacteria with an altered control of luciferase synthesis.. Journal of Bacteriology 131:519–525
    [Google Scholar]
  18. Wimpenny J. W. T., Waters P. 1984; Growth ofmicro-organisms in gel-stabilized two-dimensional diffusion gradient systems.. Journal of General Micro-biology 130:2921–2926
    [Google Scholar]
  19. Wimpenny J. W. T., Lovitt R. W., Coombs J. P. 1983; Laboratory model systems for the investigation of spatially and temporally organised microbial ecosystems.. Symposia of the Society for GeneralMicrobiology 34:67–117
    [Google Scholar]
  20. Wimpenny J. W. T., Coombs J. P., Lovitt R. W. 1984; Growth and interactions of microorganismsin spatially heterogeneous ecosystems. In Current Perspectives in Microbial Ecology PP. 291–299 Edited by Klug M. J., Reddy C. A. Washington, DC: American Society for Microbiology;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-11-2865
Loading
/content/journal/micro/10.1099/00221287-131-11-2865
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error