Lysis of by -Lactam Antibiotics: Deletion Analysis of the Role of Penicillin-binding Proteins 1A and 1B Free

Abstract

Summary: Deletions of the and genes of have been constructed and recombined into the chromosome to produce strains that completely lack penicillin-binding protein 1A or penicillin-binding protein 1B. In each case a DNA fragment internal to the gene was replaced by a fragment encoding an antibiotic resistance. The and deletions can therefore be readily introduced into other strains by P1 transduction of the antibiotic resistance. Although the complete absence of penicillin-binding protein 1A or penicillin-binding protein 1B was tolerated, the absence of both of these proteins was shown to result in bacterial lysis.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-10-2839
1985-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/10/mic-131-10-2839.html?itemId=/content/journal/micro/10.1099/00221287-131-10-2839&mimeType=html&fmt=ahah

References

  1. Broome-Smith J. K., Edelman A., Yousif S., Spratt B. G. 1985; The nucleotide sequences of the ponA and ponB genes encoding penicillin-binding proteins 1A and 1B of Escherichia coli. European Journal of Biochemistry 147:437–446
    [Google Scholar]
  2. Dretzen G., Bellard M., Sassone-Corsi P., Chambon P. 1981; A reliable method for the recovery of DNA fragments from agarose gels. Analytical Biochemistry 112:295–298
    [Google Scholar]
  3. Hashimoto-Gotoh T., Franklin F.C.H., Nordheim A., Timmis K. N. 1981; Specific purpose cloning vectors I. Low copy number, temperature-sensitive mobilization-defective pSC 101-derived containment vectors. Gene 16:227–235
    [Google Scholar]
  4. Hedge P. J., Spratt B.G. 1984; A gene fusion that localises the penicillin-binding domain of penicillin-binding protein 3 of Escherichia coli. FEBS Letters 176:179–184
    [Google Scholar]
  5. Holmes D. S., Quigley M. 1981; A rapid boiling method for the preparation of bacterial plasmids. Analytical Biochemistry 114:193–197
    [Google Scholar]
  6. Joyce C. M., Grindley N. D. F. 1984; Method for determining whether a gene of Escherichia coli is essential : application to the polA gene. Journal of Bacteriology 158:636–643
    [Google Scholar]
  7. Kato J.I., Suzuki H., Hirota Y. 1984; Overlap-ping of the coding regions for α and γ components of penicillin-binding protein 1 B in Escherichiu coli. Moleculur and General Genetics 196:449–457
    [Google Scholar]
  8. Keck W., Glauner B., Schwarz U., Broome-Smith J. K., Spratt B. G. 1985; Sequences of the active site peptides of three of the high molecular weight penicillin-binding proteins of Escherichiu coli K 12. Proceedings of the National Acadamy of sciences of the United States of America 82:1999–2003
    [Google Scholar]
  9. Lennox E. S. 1955; Transduction of linked genetic characters of the host by bacteriophage P1. Virology 01:190–206
    [Google Scholar]
  10. Matsuhashi M., Nakagawa J., Tomioka S., Ishino F., Tamaki S. 1982; Mechanism of peptido-glycan synthesis by penicillin-binding proteins in bacteria and effects of antibiotics. In Drug Resistance in Bacteria-Genetics, Biochemistry and Molecular Biology pp. 297–310 Edited by Mitsuhashi S. Tokyo Japanese: Scientific Societies Press;
    [Google Scholar]
  11. Monk M., Kinross J. 1972; Conditional lethality of recA and recB derivatives of a strain of E. coli with a temperature-sensitive DNA polymerase 1. Journal of Bacteriology 109:971–978
    [Google Scholar]
  12. Prentki P., Krisch H. M. 1984; In vitro insertional mutagenesis with a selectable DNA fragment. Gene 29:303–313
    [Google Scholar]
  13. Soberon X., Covarrubias L., Bolivar F. 1980; Construction and characterization of new cloning vehicles IV. Deletion derivatives of pBR322 and pBR325. Gene 09:287–305
    [Google Scholar]
  14. Spratt B. G. 1975; Distinct penicillin-binding proteins involved in the division, elongation and shape of Escherichia coli K 12. Proceedings of the National Acadamy of Sciences of the United States of America 72:2999–3003
    [Google Scholar]
  15. Spratt B. G. 1983; Penicillin-binding proteins and the future of β-lactam antibiotics. Journal of general Microbiology 129:1247–1260
    [Google Scholar]
  16. Spratt B. G., Jobanputra V., Schwarz U. 1977; Mutants of Escherichia coli which lack a component of penicillin-binding protein 1 are viable. FEBS Letters 79:374–378
    [Google Scholar]
  17. Spratt B. G., Boyd A., Stoker N. 1980; Defective and plaque-forming lambda transducing bacteriophage carrying penicillin-binding protein-cell shape genes. Journal of Bacteriology 143:569–581
    [Google Scholar]
  18. Stoker N. G., Fairweather N. F., Spratt B. G. 1982; Versatile low-copy-number plasmid vectors for cloning in Escherichiu coli. Gene 18:335–341
    [Google Scholar]
  19. Suzuki H., Nishimura Y., Hirota Y. 1978; On the process of cellular division in Escherichia coli: a series of mutants of E. coli altered in the penicillin-binding proteins. Proceedings of the National Acadamy of Sciences of the United States of America 75:664–668
    [Google Scholar]
  20. Tamaki S., Nakajima S., Matsuhashi M. 1977; Thermosensitive mutation in Escherichia coli simultaneously causing defects in penicillin-binding protein 1Bs and in enzyme activity for peptidoglycan synthesis in vitro. Proceedings of the National Academy of Sciences of the United States of America 74:5472–5476
    [Google Scholar]
  21. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-10-2839
Loading
/content/journal/micro/10.1099/00221287-131-10-2839
Loading

Data & Media loading...

Most cited Most Cited RSS feed