Plasmid Transfer and Behaviour in Acinetobacter calcoaceticus EBF65/65

B. A. Chopade; P. J. Wise; K. J. Towner

doi:10.1099/00221287-131-10-2805

Volume 131, Issue 10

Review Article

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Plasmid Transfer and Behaviour in Acinetobacter calcoaceticus EBF65/65

B. A. Chopade¹, P. J. Wise^1,† and K. J. Towner¹
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Affiliations: ¹ Department of Microbiology and PHLS Laboratory, University Hospital. Queen’s Medical Centre, Nottingham NG7 2UH, UK

† Present address: Department of Pure and Applied Biology, Imperial College, London SW7 2BB, UK.
Published: 01 October 1985 https://doi.org/10.1099/00221287-131-10-2805

Abstract

Summary: At least one plasmid from each of the incompatibility groups B, C, FIV, H2/S, Iα, Iδ, P, W and X was shown to be capable of transfer from Escherichia coli K12 to Acinetobacter calcoaceticus EBF65/65. Transfer was influenced by the presence of pAV2 (thought to encode a restriction-modification system) in the recipient strain; however, not all plasmids belonging to a particular incompatibility group behaved identically. All plasmids were unstable to varying degrees in A. calcoaceticus EBF65/65, but under suitable conditions were capable of transfer to further strains of EBF65/65 and re-transfer to E. coli K12. Of 40 recently isolated trimethoprim R plasmids 31 transferred successfully from E. coli K12 to A. calcoaceticus EBF65/65, but 17 of these 31 required the introduction of a second mobilizing plasmid for re-transfer to occur.