Isolation of Thermophilic Mutants of and Free

Abstract

Summary: Thermophilic mutants were isolated from mesophilic and by platinglarge numbers of cells and incubating them for several days at a temperature about 10 °C above the upper growth temperature limit for the parent mesophiles. Under these conditions we found thermophilic mutant strains that were able to grow at temperatures between 50 °C and 70 °C at a frequency of less than 10. The persistence of auxotrophic and antibiotic resistance markers in the thermophilic mutants confirmed their mesophilic origin. Transformation of genetic markers between thermophilic mutants and mesophilic parents was demonstrated at frequencies of 10 to 10 for single markers and about 10 for two unlinked markers. With the same procedure we were able to transfer the thermophilic trait from the mutant strains of to the mesophilic parental strains at a frequency of about 10, suggesting that the thermophilic trait is a phenotypic consequence of mutations in two unlinked genes.

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1985-10-01
2024-03-29
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References

  1. Baker H., Sobotka H., Hunter S. H. 1953; Growth requirements of some thermophilic and mesophilic bacilli. Journal of General Microhiology 9:485–493
    [Google Scholar]
  2. Bott K. F., Wilson G. A. 1967; Development of competence in the Bacillus suhtilis transformation system. Journal of Bacteriology 94:562–570
    [Google Scholar]
  3. Friedman S. M., Mojica T. 1978; Transformants of Bacillus subtilis capable of growth at elevated temperatures. In Biochemistry of Thermophily pp 117–126 Edited by Friedman S. M. New York: Academic Press;
    [Google Scholar]
  4. Gibson T., Gordon R. E. 1974; Endospore forming rods and cocci. In Bergey’s Manual of Determinative Bacteriology, 8th. edn pp 529–550 Edited by Buchanan R. E., Gibbons N. E. Baltimore: Williams & Wilkins;
    [Google Scholar]
  5. Gray R. J. H., Jackson H. 1973; Growth and macromolecular composition of a psychrophile, Micrococcus cryophilus, at elevated temperatures. Antonie van Leeuwenhoek 39:497–504
    [Google Scholar]
  6. Heinen U. J., Heinen W. 1972; Characteristics and properties of a caldo-active bacterium producing extracellular enzymes and two related strains. Archiv für Mikrobiologie 82:1–23
    [Google Scholar]
  7. Johnson E. J. 1979; Thermophile genetics and the genetic determinants of thermophily. In Strategies of Microbial Life in Extreme Enrironments pp 471–487 Edited by Shilo M. Weinheim: Verlag Chemie;
    [Google Scholar]
  8. Lindsay J., Creaser E. R. 1975; Enzyme thermostability is a transformable property between species. Nature, London 235:650–652
    [Google Scholar]
  9. Mcdonald W. C., Matney T. S. 1963; Genetic transfer of the ability to grow at 55 °C in Bacillus subtilis. Journal of Bacteriology 85:218–220
    [Google Scholar]
  10. Mandel M., Higa A. A. 1970; Calcium-depend-ent bacteriophage infection. Journal of Molecular Biology 53:159–162
    [Google Scholar]
  11. Normore W. M. 1976; Guanine-plus-cytosine (GC)composition of the DNA of bacteria, fungi, algae and protozoa. In Handbook of Biochemistry and Molecular Biology, 3rd. edn vol 11: p 85 Edited by Fasman G. D. Cleveland, Ohio: CRC Press;
    [Google Scholar]
  12. Saito H., Miura K. I. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochimica et biophysica acta 72:619–629
    [Google Scholar]
  13. Sharp R. J. 1982 Taxonomic and genetic studies of Bacillus thermophilus.PhD thesisTrent PolytechnicUK
    [Google Scholar]
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