Summary: Pseudomonas aeruginosa strain PAOl was compared to PAOl strains containing an IncP-l R plasmid (RPl. R68, or R68.45) in an experimental mouse burn infection model. All R plasmids tested caused a 10-to 400-fold increase in mean lethal dose (LD50). The decrease in virulence produced by plasmids R68 and R68.45 was significantly greater than the decrease caused by the closely related plasmid RPl. All plasmids also led to an increased sensitivity of strain PAOl to human serum bactericidal activity. Virulence and serum resistance of strain PAOl were restored by curing of the entire plasmid R68.45 but not by deletions in the plasmid’s transfer gene regions.
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