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Summary: Studies of the hydroxylated fatty acids in strains of Nocardia otitidis-caviarum showed variations in mycolic acid patterns, some strains containing only nocardomycolic acids and other strains having both nocardomycolic and corynomycolic acids. Mass spectroscopy showed that most of the nocardomycolic acids contained 52 to 56 carbon atoms and two double bonds in the main chain; the corynomycolic acids had saturated or monounsaturated chains and 30 to 34 carbon atoms. In the strains having both types of mycolic acid, only nocardomycolic acids were found as cell wall components.
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