1887

Abstract

Summary: Incubation of human polymorphonuclear leucocytes (HPMN) with highly purified serotype L/434/Bu elementary bodies (EB), in the presence and absence of specific antibody, resulted in a 10-fold reduction of viable count after 24 h incubation. Electron microscopy observations indicated activation of the HPMN by the EB. Attachment of the EB to the HPMN cell membrane, formation of a cytoplasmic cup and EB-containing vacuoles were observed. In addition, two types of phagocytic vacuoles were observed after 30 min incubation; in one type, a single EB was tightly surrounded by the vacuolar membrane, while the other type was enlarged and held one or more intact EB or degenerated EB or both. A fuzzy coat was observed on EB located in the HPMN vacuoles only in the presence of specific antibody. Empty vacuoles containing degenerated EB were observed in the HPMN after 24 h incubation. HPMN exposed to EB of produced a marked chemiluminescent response with a peak 14 times greater than the peak value of the control. A second stimulation with phorbol 12-myristate 13-acetate and zymosan was achieved. The chemiluminescent peak value in the presence of heat-treated EB (56 °C. 20 min) was 50% of that obtained in the presence of untreated EB. The significance of the chemiluminescent response in the killing mechanism of EB by HPMN is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-10-2627
1985-10-01
2021-07-30
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/10/mic-131-10-2627.html?itemId=/content/journal/micro/10.1099/00221287-131-10-2627&mimeType=html&fmt=ahah

References

  1. Allen R. C., Stjerholm R. L., Steel R. H. 1972; Evidence for the generation of an electronic excitation state(s) in human polymorphonuclear leukocytes and its participation in bactericidal activity. Biochemical and Biophysical Research Communications 47:679–684
    [Google Scholar]
  2. Biberfeld P. 1971; On blood lymphocytes activated by phytohaemagglutinin in vitro. Acta pathologica et microbiologica scandinavica (Supplement) 223:7–8
    [Google Scholar]
  3. Bose S. K., Paul. R. G. 1982; Purification of Chlamydia trachomatis lymphogranuloma venereum elementary bodies and their interaction with HeLa cells. Journal of General Microbiology 128:1371–1379
    [Google Scholar]
  4. Boyum A. 1968; Isolation of mononuclear cells and granulocytes from human blood. Scandinavian Journal of Clinical and Laboratory Investigation 21: (Supplement 97) 77–89
    [Google Scholar]
  5. Byrne G. I., Moulder J. W. 1978; Parasite specified phagocytes of Chlamydia psittaci and Chlamydia trachomatis by L and HeLa cells. Injection and Immunity 19:598–606
    [Google Scholar]
  6. Caldwell D. H., Kromhout J., Schachter J. 1981; Purification and characterization of the major outer membrane protein of Chlamydia trachomatis. Injection and Immunity 31:1161–1176
    [Google Scholar]
  7. Cevenini R., Rumpianesi F., Donati M., Sarov I. 1983; A rapid immunoperoxidase assay for the detection of specific IgG antibodies to Chlamydia trachomatis. Journal of Clinical Pathology 36:353–356
    [Google Scholar]
  8. Danley L. D., Hilger E. A. 1981; Stimulation of oxidative metabolism in murine polymorphonuclear leukocytes by unopsonized fungal cells: evidence for a mannose-specific mechanism. Journal of Immunology 127:551–556
    [Google Scholar]
  9. Dunlop E. M. C. 1983; Venereal disease. Chlamydial genital infection and its complications. British Journal of Hospital MedicineJanuary6–11
    [Google Scholar]
  10. Gabig I. G., Babior M. B. 1981; The killing of pathogens by phagocytes. Annual Review oj Medicine 32:313–326
    [Google Scholar]
  11. Goldstein I. M., Roos D., Kaplan H. B., Weissman G. 1975; Complement and immunoglobulins stimulate superoxide production of human leukocytes independently of phagocytosis. Journal of Clinical Investigation 56:1155–1163
    [Google Scholar]
  12. Haikin H., Sarov I. 1982; Determination of specific IgA antibodies to varicella zoster virus by immunoperoxidase assay. Journal of Clinical Pathology 35:645–649
    [Google Scholar]
  13. Hare M. J., Thin R. N. 1983; Chlamydial infection of the lower genital tract of women. British Medical Bulletin 39:138–144
    [Google Scholar]
  14. Henry-Suchet J., Catalan F., Loffredo V., Sanson M. J., Debache C., Pigeau F., Coppin R. 1981; Chlamydia trachomatis associated with chronic inflammation in abdominal specimens from women selected for tuboplasty. Fertility and Sterility 36:344–346
    [Google Scholar]
  15. Henson P. M., Johnston H. B., Spiegelberg H. L. 1972; The release of granule enzymes from human neutrophils stimulated by aggregated immunoglobulins of different classes and sub classes. Journal of Immunology 109:1182–1192
    [Google Scholar]
  16. Kimmel N., Manor E., Sarov I. 1984; A rapid immunoperoxidase assay for titration of Chlamydia trachomatis with Terasaki plates. Israel Journal of Medical Science 20:485
    [Google Scholar]
  17. MacFarlane J. T., Macrae A. D. 1983; Psittacosis. British Medical Bulletin 39:163–167
    [Google Scholar]
  18. Monnickendam M. A., Pearce J. H. 1983; Immune responses and chlamydial infections. British Medical Bulletin 39:187–193
    [Google Scholar]
  19. Moulder J. W. 1982; The relation of basic biology to pathogenic potential in the genus Chlamydia. Injection 10: Supplement 1, review 1–9
    [Google Scholar]
  20. Oriel D. J. 1983; Genital infections in men. British Medical Bulletin 39:133–137
    [Google Scholar]
  21. Paavonen J., Vesterinen E., Meyer B. 1979; Genital Chlamydia trachomatis infections in patients with cervical atypia. Obstetric Gynecology 54:289–291
    [Google Scholar]
  22. Quie P. G., Mills E. G. 1978; Microbial-phagocyte interactions. Clinics in Rheumatic Diseases15–32
    [Google Scholar]
  23. Quie P. G., Mills L. E., Holmes B. 1977; Molecular events during phagocytosis by human neutrophils. Progress in Hematology 10:1–9
    [Google Scholar]
  24. Richmond S. J., Stirling P. 1981; Localization of chlamydial group antigens in McCoy cell monolayers infected with Chlamydia trachomatis or Chlamydia psittaci. Infection and Immunity 34:561–570
    [Google Scholar]
  25. Rikihisa Y., Ito S. 1980; Localization of electron dense tracers during entry of Rickettsia tsutsugamushi into polymorphonuclear leukocytes. Infection and Immunity 30:231–243
    [Google Scholar]
  26. Rikihisa Y., Ito S. 1983; Effect of antibody on entry of Rickettsia tsutsugamushi into polymorpho nuclear leukocyte cytoplasm. Infection and Immunity 39:928–938
    [Google Scholar]
  27. Sarov I., Becker Y. 1968; RN A in the elementary bodies of trachoma agent. Nature, London 217:849–852
    [Google Scholar]
  28. Sarov I., Becker Y. 1969; Trachoma agent DNA. Journal of Molecular Biology 42:581–589
    [Google Scholar]
  29. Schachter J., Caldwell H. D. 1980; Chlamydiae. Annual Review of Microbiology 34:285–309
    [Google Scholar]
  30. Schachter J., Hill E. C., King E. B., Heilbron D. C., Ray R. M., Margolis A. J., Greenwood S. A. 1982; Chlamydia trachomatis and cervical neoplasma. Journal of the American Medical Association 248:2134–2138
    [Google Scholar]
  31. Silva S. R. L., Meirelles S. S. L., de Souza W. 1982; Mechanism of entry of Toxoplasma gondii into vertebrate cells. Journal of Submicroscopic Cytology 14:3471–482
    [Google Scholar]
  32. Soderlund G., Dahlgven C., Kihlstrom E. 1984; Interactions between human polymorphonuclear leukocytes and Chlamydia trachomatis. FEMS Microbiology Letters 2:21–25
    [Google Scholar]
  33. Trush M. A., Wilson M. E., Van Dyke K. 1978; The generation of chemiluminescence (CL) by phagocytic cells. Methods in Enzymology 57:462–494
    [Google Scholar]
  34. Walach B., Backner L. R., Boxer A. L. 1982; Review: clinical and laboratory approach to the management of neutrophil dysfunction. Israel Journal of Medical Sciences 18:897–916
    [Google Scholar]
  35. Ward M. E. 1983; Chlamydial classifications, development and structure. British Medical Bulletin 39:109–115
    [Google Scholar]
  36. Westrom L., Mardh P. A. 1982; Genital chlamydial infections in the female. In Chlamydia Infections pp. 121–139 Edited by Mardh P. A., Holmes K. K., Oriel J. D., Piot P., Schachter. J. Amsterdam, New York Oxford: Elsevier Biomedical Press;
    [Google Scholar]
  37. Westrom L., Mardh P. A. 1983; Chlamydial salpingitis. British Medical Bulletin 39:145–150
    [Google Scholar]
  38. Yong E. C., Klebanoff S. J., Kuo C. C. 1982; Toxic effect of polymorphonuclear leukocytes on Chlamydia trachomatis. Infection and Immunity 37:422–426
    [Google Scholar]
  39. Zeichner S. L. 1983; Isolation and characterization of macrophage phagosomes containing infectious and heat-inactivated Chlamydia psittaci: two phagosomes with different intracellular behaviours. Infection and Immunity 40:956–966
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-10-2627
Loading
/content/journal/micro/10.1099/00221287-131-10-2627
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error