1887

Abstract

Summary: An extracellular encystment enhancing activity (EEA) greatly stimulates cyst formation by under several different conditions. The activity appears when conditions are suboptimal for growth. EEA does not induce encystment by itself, but enhances differentiation initiated by other factors. EEA titres and their relationship to differentiation are described for encystment induced by: (1) berenil: (2) glucose starvation; and (3) total nutrient starvation. Extracellular EEA was required for maximum encystment by low density cultures in (1) and (2), but not in (3). It was required during the period of visible cyst wall formation rather than for earlier events in encystment. The effectiveness of EEA was reduced under some conditions by spontaneous changes in cellular sensitivity, and by extracellular inhibitors.

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1985-10-01
2024-04-24
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References

  1. Akins R. A. 1982; Differentiation of Acanthamoeba castellanii: evidence for a developmental morphogen and exploratory genetic approaches. PhD dissertation, The Ohio State University. Dissertation Abstracts International 423951B
    [Google Scholar]
  2. Akins R. A., Byers T. J. 1980; Differentiation promoting factors induced in Acanthamoeba by inhibitors of mitochondrial macromolecule synthesis. Developmental Biology 79126–140
    [Google Scholar]
  3. Band R. N. 1963; Extrinsic requirements for encystation by the soil amoeba, Hartmannella rhysodes. Journal of Protozoology 10101–107
    [Google Scholar]
  4. Byers T. J., Rudick V. L., Rudick M. J. 1969; Cell size, macromolecule composition, nuclear number, oxygen consumption and cyst formation during two growth phases in unagitated cultures of Acanthamoeba castellanii. Journal of Protozoology 16693–699
    [Google Scholar]
  5. Byers T.J., Akins R. A., Maynard B. J., Lefken R. A., Martin S. M. 1980; Rapid growth of Acanthamoeba in defined media; induction of encystment by glucose-acetate starvation. Journal of Protozoology 27216–219
    [Google Scholar]
  6. Chagla A. H., Griffiths A. J. 1974; Growth and encystation of Acanthamoeba castellanii. Journal of General Microbiology 85139–145
    [Google Scholar]
  7. Martin S. M., Byers T. J. 1976; Acid hydrolase activity during growth and encystment in Acanthamoeba castellanii. Journal of Protozoology 23608–613
    [Google Scholar]
  8. Neff R. J., Ray S. A., Benton W. F., Wilborn M. 1964; Induction of synchronous encystment (differentiation) in Acanthamoeba sp. Methods in Cell Physiology 0155–83
    [Google Scholar]
  9. Seilhamer J. J., Byers T. J. 1978; Mutants of Acanthamoeba castellanii resistant to erythromycin, chloramphenicol, and oligomycin. Journal of Protozoology 25486–489
    [Google Scholar]
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