Summary: A mutant of Saccharomyces cerevisiae lacking aconitase did not grow on minimal medium (MM) and had five-to tenfold less NADP+-depcndent glutamate dehydrogenase (GDH) activity than the wild-type, although its glutamine synthetase (GS) activity was still inducible. When this mutant was incubated with glutamate as the sole nitrogen source, the 2-oxoglutarate content rose, and the NADP+-dependent GDH activity increased. Furthermore, carbon-limited cultures showed a direct relation between NADP+-dependent GDH activity and the intracellular 2-oxoglutarate content. We propose that the low NADP+-dependent GDH activity found in the mutant was due to the lack of 2-oxoglutarate or some other intermediate of the tricarboxylic acid cycle.
DuboisE.,
GrensonM.,
WiameJ.M.1974; The participation of the anabolic glutamate dehydrogenase in the nitrogen catabolite repression of arginase in Saccharomyces cerevisiae. European Journal of Biochemistrr 48:603–616
FergusonA. R.,
SimsA. P.1974; The regulation of glutamine metabolism in Candida utilis: the role of glutamine in the control of glutamine synthetase. Journal of General Microbiology 80:159–171
HemmingsB. A.1978; Evidence for the degradation of nicotinamide adenine dinucleotide phosphate dependent glutamate dehydrogenase of Candida utilis during rapid enzyme inactivation. Journal of Bacteriology 133:867–877
HolzerH.,
SchneiderS.1957; Anreicherung und Trennung einer DPN-spezifischen und einer TPN-spezifischen Glutaminsaure Dehydrogenase aus Hefe. Biochemische Zeitschriji 329:361–367
KangL.,
KeelerM.,
DunlopP. C.,
RoonR. J.1982; Nitrogen catabolite repression in a glutamate auxotroph of Saccharomyces cerevisiae. Journal of Bacteriology 151:29–35
LegrainC.,
VissersS.,
DuboisE.,
LegrainM.,
WiameJ. M.1982; Regulation of glutamine synthetase from Saccharomyces cerevisiae by repression, inactivation and proteolysis. European Journal of Biochemistry 123:611–616
MazonM. J.1978; Effect of glucose starvation on the nicotinamide adenine dinucleotide phosphate dependent glutamate dehydrogenase of yeast. Journal of Bacteriology 133:780–785
MessenguyF.,
ColinD.,
Tenh AveJ. P.1980; Regulation of compartmentation of amino acid pools in Saccharomyces cerevisiae and its effects on metabolic control. European Journal of Biochemistry 108:439–445
OgurM.,
CokerL.,
OgurS.1964; Glutamate auxotrophs in Saccharomyces. I. The biochemical lesion in the gltl mutants. Biochemical and Biophysical Research Communications 141:193–197
RoonR. J.,
EvenH. L.1973; Regulation of the nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate dependent glutamate dehydrogenases of Saccharomyces cerevisiae. Journal of Bacteriology 116:367–372
SanwalB. D.,
LataM.1961; The occurrence of two different glutamic acid dehydrogenases in Neurospora crassa. Canadian Journal of Microbiology 71:319–328
SeniorP. J.1975; Regulation of nitrogen metabolism in Escherichia coli and Klebsiella aerogenes: studies with continuous-culture technique. Journal of Bacteriology 123:407–418
TachikiT.,
ToboroK.,
HorinoI.,
TochiburoT.1981; Assimilation of ammonia by Gluconobacter suhoxydans through glutamine synthetase/glutamate synthetase pathway. Agricultural and Biological Chemistry 45:1715–1717
TempestD. W., , MeersJ. L.,
BrownC. M.1970; Synthesis of glutamate in Aerobacter aerogenes by a hitherto unknown route. Biochemical Journal 117:405–407