1887

Abstract

Summary: X-ray microanalysis of thin cryosections of the cyanobacterium showed that aluminium was rapidly taken up and accumulated into polyphosphate granules. In addition, aluminium was found in the cell walls but could not be detected in the cytoplasm. The concentration of phosphorus in the medium affected the accumulation pattern; more aluminium was bound in the polyphosphate granules and in the cell walls after growth in phosphorus-rich medium. The accumulation of aluminium in these structures may function as a detoxification mechanism. Treatment with aluminium for 24 h did not cause any significant changes in the elemental composition of polyphosphate granules or cell walls.

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/content/journal/micro/10.1099/00221287-131-10-2545
1985-10-01
2021-08-03
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References

  1. Barnard T. I. 1982; Thin freeze-dried cryosections and biological X-ray microanalysis. Journal of Microscopy 126:317–332
    [Google Scholar]
  2. Foy C.D., Chaney R.L., White M.C. 1978; The physiology of metal toxicity in plants. Annual Review ol Plant Physiology 29:511–523
    [Google Scholar]
  3. Gupta B.L., Hall T. A. 1979; Quantitative electron probe X-ray microanalysis of electrolyte elements within epithelial tissue compartments. Federation Proceedings 38:144–153
    [Google Scholar]
  4. Hall T. A. 1971; The microprobe assay of chemical elements. In. Physical Techniques in Biochemical Research vol. 1A pp 157–175 Edited by Oster G. New York: Academic Press;
    [Google Scholar]
  5. Haug A. 1984; Molecular aspects of aluminium toxicity. Critical Reviews of Plant Science 1:345–373
    [Google Scholar]
  6. Jensen T. E., Baxter M., Rachlin J. W., Jani V. 1982; Uptake of heavy metals by Plectonema boryanum (Cyanophyceae) into cellular components especially polyphosphate bodies: an X-ray energy dispersive study. Environmental Pollution (Series A) 27:119–127
    [Google Scholar]
  7. Kulaev I. S., Vagabov M. 1983; Polyphosphate metabolism in microorganisms. Advances in Microbial Physiology 24:83–158
    [Google Scholar]
  8. Liljesvan B., Roomans G. M. 1976; Use of pure carbon specimen holders for analytical electron microscopy of thin sections. Ultra microscopy 2:105–107
    [Google Scholar]
  9. Morgan A. J., Davies T. W., Erasmus D. A. 1975; Analysis of droplets from iso-atomic solutions as a means of calibrating a transmission electron analytical microscope (TEAM). Journal of Microscopy 104:271–280
    [Google Scholar]
  10. PedersÉn M., Roomans G. M., AndrÉn M., Lignell A., Lindahl G., Wallström K., Forsberg Å. 1981; X-ray microanalysis of metals in algae-a contribution to the study of environmental pollution. Scanning Electron Microscopy II:499–509
    [Google Scholar]
  11. Pettersson A., HÄllbom L., Bergman B. 1985; Physiological and structural responses of the cyanobacterium Anabaena cylindrica to aluminium. Physiologia plantarum 63:153–158
    [Google Scholar]
  12. Roomans G.M. 1980a; Localization of divalent cations in phosphate-rich cytoplasmic granules in yeast. Physiologia plantarum 48:47–50
    [Google Scholar]
  13. Roomans G. M. 1980b; Quantitative X-ray micro analysis of thin sections. In X-ray Microanalysis in Biology pp 401–453 Edited by Hayat M. A. Baltimore: University Park Press;
    [Google Scholar]
  14. Roomans G. M., Kuypers G. A. J. 1980; Background determination in X-ray microanalysis of biological thin sections. Ultra microscopy 5:81–83
    [Google Scholar]
  15. Roomans G. M., SevÉus L.A. 1976; Subcellular localization of diffusible ions in the yeast Saccharomyces cerevisiae: quantitative microprobe analysis of thin freeze-dried sections. Journal of Cell Science 21:119–127
    [Google Scholar]
  16. Roomans G.M., SevÉus L. A. 1977; Preparation of thin cryosectioned standards for quantitative microprobe analysis. Journal of Sub microscopic Cytology. 9:31–35
    [Google Scholar]
  17. Roomans G. M., Wei X., Sev us L. 1982; Cryoultramicrotomy as a preparative method for X-ray microanalysis in pathology. Ultrastructural Pathology 3:65–84
    [Google Scholar]
  18. Russ J.C. 1974; The direct element ratio model for quantitative analysis of thin sections. In Microprobe Analysis as Applied to Cells and Tissues pp 269–276 Edited by Hall T., Echlin P., Kaufman R. London: Academic Press;
    [Google Scholar]
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