%0 Journal Article %A DOULL, J. %A AHMED, Z. %A STUTTARD, C. %A VINING, L. C. %T Isolation and Characterization of Streptomyces venezuelae Mutants Blocked in Chloramphenicol Biosynthesis %D 1985 %J Microbiology, %V 131 %N 1 %P 97-104 %@ 1465-2080 %R https://doi.org/10.1099/00221287-131-1-97 %I Microbiology Society, %X Twelve Streptomyces venezuelae mutants blocked in chloramphenicol biosynthesis were isolated. Two of these (Cml-1 and Cml-12) were apparently blocked in the conversion of chorismic acid to p-aminophenylalanine and three (Cml-4, Cml-5 and Cml-8) accumulated p-aminophenyl-alanine and may have been blocked in the hydroxylation reaction that converted this intermediate to p-aminophenylserine. One mutant (Cml-2) accumulated d-threo-1-p-nitro-phenyl-2-propionamido-1,3-propanediol and d-threo-1-p-nitrophenyl-2-isobutyramido-1,3-propanediol, indicating that chlorination of the α-N-acyl group of chloramphenicol was blocked. The remaining six strains did not excrete any detectable chloramphenicol pathway intermediates. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-131-1-97