@article{mbs:/content/journal/micro/10.1099/00221287-131-1-97, author = "DOULL, J. and AHMED, Z. and STUTTARD, C. and VINING, L. C.", title = "Isolation and Characterization of Streptomyces venezuelae Mutants Blocked in Chloramphenicol Biosynthesis", journal= "Microbiology", year = "1985", volume = "131", number = "1", pages = "97-104", doi = "https://doi.org/10.1099/00221287-131-1-97", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-131-1-97", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Twelve Streptomyces venezuelae mutants blocked in chloramphenicol biosynthesis were isolated. Two of these (Cml-1 and Cml-12) were apparently blocked in the conversion of chorismic acid to p-aminophenylalanine and three (Cml-4, Cml-5 and Cml-8) accumulated p-aminophenyl-alanine and may have been blocked in the hydroxylation reaction that converted this intermediate to p-aminophenylserine. One mutant (Cml-2) accumulated d-threo-1-p-nitro-phenyl-2-propionamido-1,3-propanediol and d-threo-1-p-nitrophenyl-2-isobutyramido-1,3-propanediol, indicating that chlorination of the α-N-acyl group of chloramphenicol was blocked. The remaining six strains did not excrete any detectable chloramphenicol pathway intermediates.", }