1887

Abstract

Twelve mutants blocked in chloramphenicol biosynthesis were isolated. Two of these (Cml-1 and Cml-12) were apparently blocked in the conversion of chorismic acid to -aminophenylalanine and three (Cml-4, Cml-5 and Cml-8) accumulated -aminophenyl-alanine and may have been blocked in the hydroxylation reaction that converted this intermediate to -aminophenylserine. One mutant (Cml-2) accumulated --1--nitro-phenyl-2-propionamido-1,3-propanediol and --1--nitrophenyl-2-isobutyramido-1,3-propanediol, indicating that chlorination of the α--acyl group of chloramphenicol was blocked. The remaining six strains did not excrete any detectable chloramphenicol pathway intermediates.

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/content/journal/micro/10.1099/00221287-131-1-97
1985-01-01
2021-05-18
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