1887

Abstract

The nitro radical-anion of metronidazole has been detected in the sexually transmitted human parasite, , under anaerobic conditions by electron spin resonance spectrometry. Exposure of organisms to oxygen decreased the intensity of the radical signal in both metronidazole-sensitive ATCC strain 30001 and in the metronidazole-resistant strain 85. The sensitive strain still gave radical signals at partial pressures of oxygen (>6 kPa) sufficient to remove all detectable radicals from the resistant strain. This evidence suggests that the resistant strain has defective oxygen scavenging system(s).

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1985-01-01
2021-07-29
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References

  1. Bailar J. C. Jr, Jones E. M. 1935; Trioxalato salts. (Trioxalato-aluminiate, -ferriate, -chromiate and -cobaltiate). Inorganic Synthesis 1:35–38
    [Google Scholar]
  2. Beaulieu B. B. Jr, McLafferty M. A., Koch R. L., Goldman P. 1981; Metronidazole metabolism in cultures of Entamoeba histolytica and Trkhomonas vaginalis. Antimicrobial Agents and Chemotherapy 20:410–414
    [Google Scholar]
  3. Berg S. P., Nesbitt D. M. 1979; Chromium Oxalate: A new spin label broadening agent for use with thylakoids. Biochimica et biophysica acta 548:608–615
    [Google Scholar]
  4. Breccia A., Cavalleri B., Adams G. E. 1982 Nitroimidazoles; Chemistry, Pharmacology and Clinical Applications New York: Plenum;
    [Google Scholar]
  5. Čerkasovová A. J., Cerkasov J., Kulda J., Demes P. 1980; Metronidazole action on Tritrichomonas foetus: enzyme activities in the strain of a different resistance to the drug. In The Host-Invader Interplay669–672 Van den Bossche H. Amsterdam: Elsevier;
    [Google Scholar]
  6. Clackson T. E., Coombs G. H. 1982; Factors affecting the trichomonacidal activity of metronidazole. Journal of Protozoology 29:636
    [Google Scholar]
  7. Diamond L. 1957; The establishment of various trichomonads of animals and man in axenic cultures. Journal of Parasitology 43:488–490
    [Google Scholar]
  8. Edwards D. I. 1979; Mechanism of antimicrobial action of metronidazole. Journal of Antimicrobial Chemotherapy 5:499–502
    [Google Scholar]
  9. Edwards D. I., Dye M., Carne H. 1973; The selective toxicity of antimicrobial nitroheterocyclic drugs. Journal of General Microbiology 76:135–145
    [Google Scholar]
  10. Ings R. M. J., McFadzean J. A., Omerod W. E. 1974; The mode of action of metronidazole in Trichomonas vaginalis and other microorganisms. Biochemical Pharmacology 23:1421–1429
    [Google Scholar]
  11. Jørgensen L., Johansen T. 1983; Affinity of drugs for cytochrome P-450 determined by inhibition of p-nitrophenetole O-de-ethylation by rat liver microsomes. Ada pharmacologica et toxicologica 53:70–77
    [Google Scholar]
  12. Lloyd D., Ohnishi T., Lindmark D. G., Müller M. 1983; Respiration of Tritrichomonas foetus. Wiadomosci Parazytologiczne 29:37–39
    [Google Scholar]
  13. Marczak R., Gorrell T. E., Müller M. 1983; Hydrogenosomal ferredoxin of the anaerobic protozoon, Tritrichomonas foetus. Journal of Biological Chemistry 258:12427–12433
    [Google Scholar]
  14. Mason R. P. 1979; Free radical metabolites of foreign compounds and their toxicological significance. In Reviews in Biochemical Toxicology151–200 Hodgson E., Bend J. R., Philpot R. M. Amsterdam, Holland: Elsevier;
    [Google Scholar]
  15. Mason R. P., Holtzman J. L. 1975; The mechanism of microsomal and mitochondrial nitro-reductase. Electron spin resonance evidence for nitroaromatic free radical intermediates. Biochemistry 14:1626–1632
    [Google Scholar]
  16. Meingassner J. G., Thurner J. 1979; Strain of Trichomonas vaginalis resistant to metronidazole and other 5-nitroimidazoles. Antimicrobial Agents and Chemotherapy 15:254–257
    [Google Scholar]
  17. Meingassner J. G., Mieth H., Czok R., Lindmark D. G., Müller M. 1978; Assay conditions and the demonstration of nitroimidazole resistance in Tritrichomonas foetus. Antimicrobial Agents and Chemotherapy 13:1–3
    [Google Scholar]
  18. Milne S. E., Stokes E. J., Waterworth P. M. 1978; Incomplete anaerobiosis as a cause of metronidazole ‘resistance’. Journal of Clinical Pathology 31:933–935
    [Google Scholar]
  19. Moreno S. N. J., Mason R. P., Muniz R. P. A., Cruz F. S., Docampo R. 1983; Generation of free radicals from metronidazole and other nitroimi-dazoles by Tritrichomonas foetus. Journal of Biological Chemistry 258:4051–4054
    [Google Scholar]
  20. Müller M., Gorrell T. E. 1984; Biochemical characteristics of Trichomonas vaginalis isolates with differing metronidazole susceptibility. Antimicrobial Agents and Chemotherapy (in the Press)
    [Google Scholar]
  21. Müller M., Lindmark D. G. 1976; Uptake of metronidazole and its effect on viability in trichomonads and Entamoeba invadens under anaerobic and aerobic conditions. Antimicrobial Agents and Chemotherapy 9:696–700
    [Google Scholar]
  22. Müller M., Meingassner J. G., Miller W. A., Ledger W. J. 1980; Three metronidazole-resistant strains of Trichomonas vaginalis from the United States. American Journal of Obstetrics and Gynecology 138:808–812
    [Google Scholar]
  23. Ohnishi T., Lloyd D., Lindmark D. G., Müller M. 1980; Respiration of Tritrichomonas foetus: Components detected in Hydrogenosomes and in intact cells by electron paramagnetic resonance spectrometry. Molecular and Biochemical Parasitology 2:39–50
    [Google Scholar]
  24. Perez-Reyes E., Kalyanaraman B., Mason R. P. 1980; The reductive metabolism of metronidazole and ronidazole by aerobic liver microsomes. Molecular Pharmacology 17:239–244
    [Google Scholar]
  25. Wardman P., Clarke E. D. 1976; Oxygen inhibition of nitroreductase: Electron transfer from nitro-radical-anions to oxygen. Biochemical and Biophysical Research Communications 69:942–949
    [Google Scholar]
  26. Yarlett N., Gorrell T. E., Marczak R., Müller M. 1984; Reduction of nitroimidazole derivatives by hydrogenosomal extracts of Trichomonas vaginalis. Molecular and Biochemical Parasitology (in the Press)
    [Google Scholar]
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