1887

Abstract

The dual energy requirement (protonmotive force and ATP) of the Trk potassium transport system has been investigated. Using inhibitors and mutants we show that Trk is not an ATPase but may be regulated by ATP. Possible mechanisms of energy coupling to Trk are discussed.

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/content/journal/micro/10.1099/00221287-131-1-77
1985-01-01
2021-07-27
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References

  1. Bakker E. P. 1980; The role of ATP and the proton motive force in potassium transport by the Escherichia coli TrkA system. 315–318In Vectorial European Bioenergetics Conference283–284
    [Google Scholar]
  2. Bakker E. P. 1981; The role of ATP and the proton motive force in potassium transport by the Escherichia coli TrkA system. 315–318 In Vectorial Reactions in Electron and Ion Transport in Bacteria315–318 Palmieri F., Quagliariello E., Siliprandi N., Slater E. C. Amsterdam: Esevier/ North Holland Biomedical Press;
    [Google Scholar]
  3. Bakker E. P., Harold F. M. 1980; Energy coupling to potassium transport in Streptococcus faecalis. Interplay of ATP and the protonmotive force. Journal of Biological Chemistry 255:433–440
    [Google Scholar]
  4. Berger E. A. 1973; Different mechanisms of energy coupling for the active transport of proline and glutamine in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 701514–1518
    [Google Scholar]
  5. Berger E. A., Heppel L. A. 1974; Different mechanisms of energy coupling for the shock sensitive and shock resistant amino acid permeases of Escherichia coli. Journal of Biological Chemistry 249:7747–7755
    [Google Scholar]
  6. Elferinck M. G. L., Hellingwerf K. J., Belkum M. J., Poolman B., Konings W. N. 1984; Direct interaction between linear electron transfer chains and solute transport systems in bacteria. FEMS Microbiology Letters 21:293–298
    [Google Scholar]
  7. Epstein W., Kim B. S. 1971; Potassium transport loci in Escherichia coli. Journal of Bacteriology 108:639–644
    [Google Scholar]
  8. Epstein W., Schultz S. G. 1965; Cation transport in Escherichia coli. V. Regulation of cation content. Journal of General Physiology 49:221–234
    [Google Scholar]
  9. Epstein W., Whitelaw V., Hesse J. 1978; A K+ transport ATPase in Escherichia coli. Journal of Biological Chemistry 253:6666–6668
    [Google Scholar]
  10. Harold F. M., Altendorf K. 1974; Cation transport in bacteria: K+, Na+ and H+ . Current Topics in Membranes and Transport 5:1–50
    [Google Scholar]
  11. Helmer G. L., Epstein W. 1981; Rate limiting components of Trk mediated potassium uptake by Escherichia coli K-12. Biophysical Journal 33:61a
    [Google Scholar]
  12. Helmer G. L., Laimins L. A., Epstein W. 1982; Mechanisms of potassium transport in bacteria. In Membranes and Transport 2123–128 Martinosi A. N. New York: Plenum Press;
    [Google Scholar]
  13. Kimmich G. A., Randles J., Brand J. S. 1975; Assay of picomole amounts of ATP, ADP and AMP using the luciferase enzyme system. Analytical Biochemistry 69:187–206
    [Google Scholar]
  14. Kroll R. G., Booth I. R. 1981; The role of potassium transport in the generation of a pH gradient in Escherichia coli. Biochemical Journal 198:691–698
    [Google Scholar]
  15. Laimins L., Rhoads D. B., Altendorf K., Epstein W. 1978; Identification of structural proteins of an ATP-driven potassium transport system in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 753216–3219
    [Google Scholar]
  16. Leive L. 1968; Studies on the permeability change produced in coliform bacteria by ethylenediamine-tetraacetate. Journal of Biological Chemistry 243:2373–2380
    [Google Scholar]
  17. Lubin M., Ennis H. L. 1964; On the role of intracellular potassium in protein synthesis. Biochimica et biophysica acta 80:614–631
    [Google Scholar]
  18. Plack R. H., Rosen B. P. 1980; Cation/proton antiport systems in Escherichia coli. Absence of potassium/proton antiporter activity in a pH-sensitive mutant. Journal of Biological Chemistry 255:3824–3825
    [Google Scholar]
  19. Rhoads D. B., Epstein W. 1977; Energy coupling to net K+ transport in Escherichia coli K-12. Journal of Biological Chemistry 253:1394–1401
    [Google Scholar]
  20. Rhoads D. B., Epstein W. 1978; Cation transport in Escherichia coli. IX. Regulation of K+ transport. Journal of General Physiology 72:283–295
    [Google Scholar]
  21. Rhoads D. B., Waters F. B., Epstein W. 1976; Cation transport in Escherichia coli. VIII. Potassium transport mutants. Journal of General Physiology 67:325–341
    [Google Scholar]
  22. Rosen B. P. 1973; Restoration of active transport in an Mg2+ ATPase deficient mutant of Escherichia coli. Journal of Bacteriology 116:1124–1129
    [Google Scholar]
  23. Rosen B. P., Kashket E. R. 1978; Energetics of active transport. In Bacterial Transport559–612 Rosen B. P. New York: Dekker;
    [Google Scholar]
  24. Rosen B. P., Brey R. N., Hasan S. H. 1978; Energy transduction in Escherichia coli: New mutation affecting the F0 portion of the ATP synthetase complex. Journal of Bacteriology 134:1030–1038
    [Google Scholar]
  25. Suelter C. H. 1970; Enzymes activated by monovalent cations. Science 168:789–795
    [Google Scholar]
  26. Stewart L. M. D., Booth I. R. 1983; Na+ involvement in proline transport in Escherichia coli. FEMS Microbiology Letters 19:161–164
    [Google Scholar]
  27. West I. C., Mitchell P. 1973; Stoichiometry of lactose-proton symport across the plasma membrane of Escherichia coli. Biochemical Journal 132:587–592
    [Google Scholar]
  28. Wieczorek L., Altendorf K. 1979; K+-transport adenosine-5′-triphosphatase. FEBS Letters 98:233–236
    [Google Scholar]
  29. Zilberstein D., Agmon V., Schuldiner S., Padan E. 1982; The sodium/proton antiporter is part of pH homeostasis mechanism in Escherichia coli. Journal of Biological Chemistry 257:3687–3691
    [Google Scholar]
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