1887

Abstract

When was grown in batch culture in the presence of various Tween detergents, the fatty acid moiety of the detergent was incorporated into the lipids of its membrane. Tween 80 (containing primarily oleic acid) markedly stimulated the production of extracellular glucosyltransferase and also increased the degree of unsaturation of the membrane lipid fatty acids. The possibility that an increase in membrane unsaturated fatty acids promoted extracellular glucosyltransferase production was examined by growing cells at different temperatures in the presence or absence of Tween 80. The membrane lipids of cells grown at 30°C, 37°C and 40°C without Tween 80 exhibited unsaturated/saturated fatty acid ratios of 2·06, 1·01 and 0·87 respectively. A significant increase in the production of extracellular glucosyltransferase was observed at 30°C compared to cells grown at 40°C. However, cells produced much more exoenzyme at all temperatures when grown with Tween 80. The results indicated that an increase in the unsaturated fatty acid content of the membrane lipids was not by itself sufficient to account for the stimulation of extracellular glucosyltransferase production by Tween 80, but that the surfactant also had to be present.

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1985-01-01
2022-01-22
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References

  1. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37:911–917
    [Google Scholar]
  2. Carlsson J., Elander B. 1973; Regulation of dextransucrase formation by Streptococcus sanguis . Caries Research 7:81–101
    [Google Scholar]
  3. Cronan J. E., Gelmann E. P. 1975; Physical properties of membrane lipids : biological relevance and regulation. Bacteriological Reviews 39:232–256
    [Google Scholar]
  4. Daniels C. J., Bole D. G., Quay S. C., Oxender D. L. 1981; Role for membrane potential in the secretion of protein into the periplasm of Escherichia coli . Proceedings of the National Academy of Sciences of the United States of America 785396–5400
    [Google Scholar]
  5. Enequist H. G., Hirst T. R., Harayama S., Hardy S. J. S., Randall L. L. 1981; Energy is required for maturation of exported proteins in Escherichia coli . European Journal of Biochemistry 116:227–233
    [Google Scholar]
  6. Evkokimova O. A., Nesmeyanova M. A., Kulaev I. S. 1978; Indikutsiia sinteza shehelochnoi fosfatazy u E. coli preinkubatsieri kletok pri ponizhenno temperatyre. Biokhimiya 43:1680–1687
    [Google Scholar]
  7. Fishman Y., Rottem S., Citri N. 1980; Preferential suppression of normal exoenzyme formation by membrane-modifying agents. Journal of Bacteriology 141:1435–1438
    [Google Scholar]
  8. Gibbons R. J., Banghart S. B. 1967; Synthesis of extracellular dextran by cariogenic bacteria and its presence in human dental plaque. Archives of Oral Biology 12:11–24
    [Google Scholar]
  9. Gibbons R. J., Fitzgerald R. J. 1969; Dextran induced agglutination of Streptococcus mutans and its potential role in the formation of microbial dental plaques. Journal of Bacteriology 98:341–346
    [Google Scholar]
  10. Gibbons R. J., van Houte J. 1975; Bacterial adherence in oral microbial ecology. Annual Review of Microbiology 29:19–44
    [Google Scholar]
  11. Hamilton I. R., Phipps P. J., Ellwood D. C. 1979; Effect of growth rate and glucose concentration on the biochemical properties of Streptococcus mutans Ingbritt in continuous culture. Infection and Immunity 26:861–869
    [Google Scholar]
  12. Hardy L., Jacques N. A., Forester H., Campbell L. K., Knox K. W., Wicken A. J. 1981; Effect of fructose and other carbohydrates on the surface properties, lipoteichoic acid production and extracellular proteins of Streptococcus mutans Ingbritt grown in continuous culture. Infection and Immunity 31:78–87
    [Google Scholar]
  13. Hoffmann K., Tausig F. 1955; The chemical nature of the fatty acids of a Group C streptococcus species. Journal of Biological Chemistry 213:415–423
    [Google Scholar]
  14. Jacques N. A. 1982; Relationship between cyclopropane synthetase and the formation of cyclopropane fatty acids by Proteus vulgaris grown under various respiratory conditions. Journal of General Microbiology 128:177–184
    [Google Scholar]
  15. Jacques N. A. 1983; Membrane perturbation by cerulenin modulates glucosyltransferase secretion and acetate uptake by Streptococcus salivarius . Journal of General Microbiology 129:3293–3302
    [Google Scholar]
  16. Janda W. M., Kuramitsu H. 1976; Regulation of extracellular glucosyltransferase production and the relationship between extracellular and cell-associated activities in Streptococcus mutans . Infection and Immunity 14:191–202
    [Google Scholar]
  17. Keevil C. W., West A. A., Bourne N., Marsh P. D. 1984; Inhibition of the synthesis of extracellular glucosyl- and fructosyltransferase in Streptococcus sanguis by sodium ions. Journal of General Microbiology 130:77–82
    [Google Scholar]
  18. Kimura K., Izui K. 1976; Importance of membrane fluidity in the induction of alkaline phosphatase, a periplasmic enzyme, in Escherichia coli . Biochemical and Biophysical Research Communications 70:900–906
    [Google Scholar]
  19. Kito M., Ishimaga M., Nishihara M., Kato M., Sawada S., Hata T. 1975; Metabolism of the phosphatidylglycerol molecular species in Escherichia coli . European Journal of Biochemistry 54:55–63
    [Google Scholar]
  20. Marr A. G., Ingraham J. L. 1962; Effect of temperature on the composition of fatty acids in Escherichia coli . Journal of Bacteriology 84:1260–1267
    [Google Scholar]
  21. de Mendoza D., Cronan J. E. 1983; Thermal regulation of membrane lipid fluidity in bacteria. Trends in Biochemical Sciences 8:49–52
    [Google Scholar]
  22. Nesmeyanova M. A. 1982; On the possible participation of acid phospholipids in the translocation of secreted proteins through the bacterial cytoplasmic membrane. FEBS Letters 142:189–193
    [Google Scholar]
  23. Pages J. M., Lazdunski C. 1982; Membrane potential depolarizing agents inhibit maturation of exported proteins in Escherichia coli . FEBS Letters 149:51–54
    [Google Scholar]
  24. Petit-Glatron M.-F., Chambert R. 1981; Levansucrase of Bacillus subtilis : conclusive evidence that its production and export are unrelated to fatty acid synthesis but modulated by membrane-modifying agents. European Journal of Biochemistry 119:603–611
    [Google Scholar]
  25. Robrish S. A., Reid W., Krichevsky M. I. 1972; Distribution of enzymes forming polysaccharides from sucrose and the composition of extracellular polysaccharide synthesized by Streptococcus mutans . Applied Microbiology 24:184–190
    [Google Scholar]
  26. Schachtele C. F., Harlander S. K., Germaine G. R. 1976; Streptococcus mutans dextransucrase: availability of disaggregated enzyme after growth in a chemically defined medium. Infection and Immunity 13:1522–1524
    [Google Scholar]
  27. Umesaki Y., Kawai Y., Mutai M. 1977; Effect of Tween 80 on glucosyltransferase production in Streptococcus mutans . Applied and Environmental Microbiology 34:115–119
    [Google Scholar]
  28. Wittenberger C. L., Beaman A. J., Lee L. N. 1978; Tween 80 effect on glucosyltransferase synthesis by Steptococcus salivarius . Journal of Bacteriology 133:231–239
    [Google Scholar]
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