1887

Abstract

The presence and location of an acetolysis-resistant polymer (sporopollenin) in various fruiting structures of the cellular slime moulds were demonstrated. Macrocysts (giant cells with thick cell walls) formed by a mutant (MF-1) of 7 contained large amounts of sporopollenin in their walls. In contrast, microcysts (round, walled resting cells) of ws-320 lacked sporopollenin, at least in a tight networked form. This difference in sporopollenin distribution seems to be at least partly related to a difference in the germination efficiency of the two types of cells. In fruiting bodies, the acellular stalk was found to consist mainly of sporopollenin, but the spores lacked it in a tight networked form. Spores of and contained sporopollenin in the wall region. All the above species belong to the class Dictyostelia. Fruiting bodies of a member of the class Acrasea, lack a tight network of cellulose wall as well as sporopollenin. It is clear that slime mould sporopollenin shows unique distribution patterns depending upon cell type and species. The functional significance of sporopollenin is discussed, with special reference to the late developmental stages of slime mould cells.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-1-201
1985-01-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/1/mic-131-1-201.html?itemId=/content/journal/micro/10.1099/00221287-131-1-201&mimeType=html&fmt=ahah

References

  1. Amagai A., Filosa M. F. 1984; The involvement of cyclic AMP and volatile substance(s) in the development of a macrocyst-forming strain of Dictyostelium mucoroides . Development, Growth and Differentiation (in the Press)
    [Google Scholar]
  2. Brooks J., Grant P. R., Mum M. D., van Gijzel P., Shaw G. 1971 Sporopollenin London & New York: Academic Press;
    [Google Scholar]
  3. Erdtman G. 1960; The acetolysis method. A revised description. Svensk botanish tidskrift 54:561–564
    [Google Scholar]
  4. Francis D. W. 1979; True divergent differentiation in a cellular slime mold Polysphondylium pallidum . Differentiation 15:187–192
    [Google Scholar]
  5. MacInnes M. A., Francis D. W. 1974; Meiosis in Dictyostelium mucoroides . Nature London: 251321–323
    [Google Scholar]
  6. Maeda Y. 1984; The presence and location of sporopollenin in fruiting bodies of the cellular slime moulds. Journal of Cell Science 66:297–308
    [Google Scholar]
  7. O’Day D. H. 1974; Intracellular and extracellular enzyme patterns during microcyst germination in the cellular slime mold Polysphondylium pallidum . Developmental Biology 36:400–410
    [Google Scholar]
  8. Olive L. S. 1975 The Mycetozoans New York, San Francisco & London: Academic Press;
    [Google Scholar]
  9. Olive L. S., Stoianovitch C. 1960; Two new members of the Acrasiales. Bulletin of the Torrey Botanical Club 87:1–20
    [Google Scholar]
  10. Raper K. B., Quinlan M. S. 1958; Acytostelium leptosomum: a unique cellular slime mould with an acellular stalk. Journal of General Microbiology 18:16–32
    [Google Scholar]
  11. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17:208–212
    [Google Scholar]
  12. Shaw G. 1971 In Sporopollenin305–348 Brooks J., Grant P. R., Muir M. D., van Gijzel P., Shaw G. London & New York: Academic Press;
    [Google Scholar]
  13. Toama M. A., Raper K. B. 1967; Microcysts of the cellular slime mold Polysphondylium pallidum. I. Factors influencing microcyst formation. Journal of Bacteriology 94:1143–1149
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-1-201
Loading
/content/journal/micro/10.1099/00221287-131-1-201
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error