@article{mbs:/content/journal/micro/10.1099/00221287-131-1-17, author = "BROWNLIE, R. M. and PARTON, R. and COOTE, J. G.", title = "The Effect of Growth Conditions on Adenylate Cyclase Activity and Virulence-related Properties of Bordetella pertussis", journal= "Microbiology", year = "1985", volume = "131", number = "1", pages = "17-25", doi = "https://doi.org/10.1099/00221287-131-1-17", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-131-1-17", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Growth of Bordetella pertussis in Stainer & Scholte medium in which the NaCl had been replaced by one of several inorganic or organic salts resulted in a large decrease in adenylate cyclase activity, histamine-sensitizing activity and in the amounts of two cell-envelope polypeptides of M r 28000 and 30000. Although some variation between strains was observed, there was never a case where one of these properties was lost independently of the others. Cultures in which these properties were lost had decreased amounts of extracellular cAMP when compared to NaCl-grown cultures. Adenylate cyclase activity was detected in three locations of B. pertussis cultures (extracellular, extracytoplasmic but cell-associated, and cytoplasmic). After growth in medium containing high concentrations of MgSO4, enzyme activity was decreased to a similar extent in all three locations.", }