1887

Abstract

A mutation in a strain of 15 produced ribosomes by an abnormal pathway that caused the accumulation of 47S ribonucleoprotein particles. The mutation was transferred to strains of K12 by transductions with bacteriophage Plcam and was at about 82 min, between and and rather closer to the latter. The location and the physiological properties of the mutant suggested that the mutation was in the transcription unit and affected the synthesis of ribosomal proteins L28 and L33.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-1-135
1985-01-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/1/mic-131-1-135.html?itemId=/content/journal/micro/10.1099/00221287-131-1-135&mimeType=html&fmt=ahah

References

  1. Arber W., Wauters-Willems D. 1970; Host specificity of DNA produced by Escherichia coli. XII. The two restriction and modification systems of strain 15T. Molecular and General Genetics 108:203–217
    [Google Scholar]
  2. Barritault D., Expert-Bezancon A., Guerin M.-F., Hayes D. 1976; The use of acetone precipitation in the isolation of ribosomal proteins. European Journal of Biochemistry 63:131–135
    [Google Scholar]
  3. Blundell M. R., Wild D. G. 1969; Inhibition of bacterial growth by metal salts. The accumulation of ribonucleic acid during inhibition of Escherichia coli by cobalt chloride. Biochemical Journal 115:213–223
    [Google Scholar]
  4. Butler P. D., Sims P. F. G., Wild D. G. 1978; Binding of chloramphenicol and a fragment of aminoacyl-transfer ribonucleic acid to ribosomes and a ribosome precursor from a mutant of Escherichia coli . Biochemical Journal 172:503–508
    [Google Scholar]
  5. Butler P. D., Sims P. F. G., Wild D. G. 1980; Intermediates in the assembly of ribosomes by a mutant of Escherichia coli . Biochemical Journal 190:157–170
    [Google Scholar]
  6. Essenberg R. C., Kornberg H. L. 1977; Location of the gene specifying hexose phosphate transport (uhp) on the chromosome of Escherichia coli. Journal of General Microbiology 99:157–169
    [Google Scholar]
  7. Gallant J. A. 1979; Stringent control in E. coli. Annual Reviews of Genetics 13:393–415
    [Google Scholar]
  8. Hardy S. J. S. 1975; The stoichiometry of the ribosomal proteins of Escherichia coli. Molecular and General Genetics 14:253–274
    [Google Scholar]
  9. Isono K., Schnier J., Kitakawa M. 1980; Genetic fine structure of the pyrE region containing the genes for ribosomal proteins L28 and L33 in Escherichia coli. Molecular and General Genetics 179:311–317
    [Google Scholar]
  10. Jinks-Robertson S., Gourse R. L., Nomura M. 1983; Expression of rRNA and tRNA genes in Escherichia coli: evidence for feedback regulation by products of rRNA operons. Cell 33:865–876
    [Google Scholar]
  11. Kenny J. W., Lambert J. M., & Traut R. R. 1979; Cross-linking of ribosomes using 2-iminothiolane (methyl 4-mercaptobutyrimidate) and identification of cross-linked proteins by diagonal polyacrylamide/ sodium dodecyl sulfate gel electrophoresis. Methods in Enzymology 59:534–550
    [Google Scholar]
  12. Konrad E. B., Lehman I. R. 1975; Novel mutants of Escherichia coli that accumulate very small DNA replicative intermediates. Proceedings of the National Academy of Sciences of the United States of America 722150–2154
    [Google Scholar]
  13. Lee J. S., An G., Friesen J. D., Isono K. 1981; Cloning and the nucleotide sequence of the genes for Escherichia coli ribosomal proteins L28 (rpmB) and L33 (rpmG). Molecular and General Genetics 184:218–223
    [Google Scholar]
  14. Lindhal L., Zengel J. M. 1982; Expression of ribosomal genes in bacteria. Advances in Genetics 21:53–121
    [Google Scholar]
  15. Lindahl L., Archer R., Zengel J. M. 1983; Transcription of the S10 ribosomal protein operon is regulated by an attenuator in the leader. Cell 33:241–248
    [Google Scholar]
  16. MacDonald R. E., Turnock G., Forchhammer J. 1967; The synthesis and function of ribosomes in a new mutant of Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 57141–147
    [Google Scholar]
  17. Madjar J.-J., Michel S., Cozzone A. J., Reboud J.-P. 1979; A method to identify individual proteins in four different two-dimensional gel electrophoresis systems: application to Escherichia coli ribosomal proteins. Analytical Biochemistry 92:174–192
    [Google Scholar]
  18. Markey F., Wild D. G. 1975; A 30S precursor of 30S ribosomes in a mutant of Escherichia coli. Biochemical Journal 151:463–465
    [Google Scholar]
  19. Markey F., Wild D. G. 1976; An unusual precursor of 50S ribosomes in a mutant of Escherichia coli. Biochemical Journal 154:311–318
    [Google Scholar]
  20. Markey F., Sims P. F. G., Wild D. G. 1976; The composition of an unusual precursor of 50S ribosomes in a mutant of Escherichia coli. Biochemical Journal 158:451–456
    [Google Scholar]
  21. Rosner J. L. 1972; Formation, induction and curing of bacteriophage P1 lysogens. Virology 49:679–689
    [Google Scholar]
  22. Turnock G. . 1969; A genetic analysis of a mutant of Escherichia coli with a defect in the assembly of ribosomes. Molecular and General Genetics 104:295–312
    [Google Scholar]
  23. Yamamoto K. R., Alberts B. M., Benzinger R., Lawthorne L., Treiber G. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology 40:734–744
    [Google Scholar]
  24. Yudkin M. D. 1976; Mutations in Escherichia coli that relieve catabolite repression of tryptophanase synthesis. Mutations distant from the tryptophanase gene. Journal of General Microbiology 92:125–132
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-1-135
Loading
/content/journal/micro/10.1099/00221287-131-1-135
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error