1887

Abstract

After trichloroacetic acid extraction of the walls of alkalophilic sp. strain C-125 grown at an alkaline or a neutral pH, two acidic structural polymer fractions were isolated by ionexchange chromatography and gel chromatography. One was a teichuronic acid fraction containing -acetyl--fucosamine, glucuronic acid and galacturonic acid in a molar ratio of 1: 1: 1. This teichuronic acid amounted to 390 μg per mg peptidoglycan in the walls of the bacterium grown at an alkaline pH or 80 μg per mg at a neutral pH. The molecular weight of the teichuronic acid from the cells grown at an alkaline pH was 70000 compared to a molecular weight of 48000 at a neutral pH as estimated by gel chromatography. The other fraction contained glucuronic acid and -glutamic acid in a molar ratio of 1: 5. This fraction was either a copolymer of both the acids or a mixture of polyglucuronate and polyglutamate. This fraction amounted to 560 μg per mg peptidoglycan in the walls of the bacterium grown at an alkaline pH or 240 μg per mg at a neutral pH. The molecular weight of the fraction from both the wall preparations was about 19000.

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/content/journal/micro/10.1099/00221287-131-1-105
1985-01-01
2024-11-14
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References

  1. Aono R., Horikoshi K. 1982; Chemical composition of cell walls of alkalophilic strains of Bacillus. Journal of General Microbiology 129:1083–1087
    [Google Scholar]
  2. Aono R., Horikoshi K., Goto S. 1984; Com-position of the peptidoglycan of alkalophilic Bacillus spp. Journal of Bacteriology 157:688–689
    [Google Scholar]
  3. Ashwell G. 1957; Colorimetric analysis of sugars. Methods in Enzymology 3:73–105
    [Google Scholar]
  4. Horikoshi K., Akiba T. 1982; Cell surface. In Alkalophilic Microorganisms75–77 Heidelberg: Springer-Verlag;
    [Google Scholar]
  5. Park J. T., Johnson M. J. 1949; A submicro-determination of glucose. Journal of Biological Chemistry 181:149–151
    [Google Scholar]
  6. Perkins H. R. 1965; The action of hot formamide on bacterial cell walls. Biochemical Journal 95:876–882
    [Google Scholar]
  7. White P. J., Gilvarg C. 1977; A teichuronic acid containing rhamnose from cell walls of Bacillus megaterium. Biochemistry 16:2428–2435
    [Google Scholar]
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