Purification, Characterization and Immunological Properties of the Serotype-specific Capsular Polysaccharide of (Serotype A1) Organisms Free

Abstract

The serotype-specific capsular polysaccharide from two strains of serotype A1 organisms was purified and characterized by chemical analysis and NMR spectroscopy. The polymer has the structure →3)--(2-acetamido-2-deoxy-4--acetyl--mannopyranosyluronic acid)-(1→4)--(2-acetamido-2-deoxy--mannopyranose)-(1→. The polysaccharide was immunogenic (able to evoke production of antibodies) for sheep but not for rabbits. Immuno electron-microscopy studies using the Protein A-gold technique showed the polysaccharide to be peripherally located on the bacterial surface. Reduction, oxidation and de--acetylation of the polymer did not appear to alter its immunological precipitability with specific antiserum, but all three treatments destroyed its ability to adhere to sheep erythrocytes at neutral pH. De--acetylation of the polymer destroyed both immunological precipitability and erythrocyte adherence.

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1984-09-01
2024-03-28
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References

  1. Beesley J. E., Orpin A., Adlam C. 1982; A comparison of immunoferritin, immuno-enzyme and gold-labelled protein A methods for the localisation of capsular antigen on frozen thin sections of the bacterium Pasteurella haemolytica. Histochemical Journal 14:803–810
    [Google Scholar]
  2. Bennett L. G., Bishop C. T. 1980; Structure of the type XXXIII Streptococcus pneumoniae (pneumococcal) capsular polysaccharide. Canadian Journal of Chemistry 58:2724–2727
    [Google Scholar]
  3. Bhattacharjeb A. K., Jennings H. J., Kenny C. P., Martin A., Smith I. C. P. 1975; Structural determination of the sialic acid polysaccharide antigens of Neisseria meningitidis serogroups B and C with carbon 13 nuclear magnetic resonance. Journal of Biological Chemistry 250:1926–1932
    [Google Scholar]
  4. Bock K., ThØgerson H. 1982; Nuclear magnetic resonance spectroscopy in the study of mono and polysaccharides. Annual Reports of NMR Spectroscopy 13:1–57
    [Google Scholar]
  5. Bundle D. R., Jennings H. J., Smith I. C. P. 1973; The carbon-13 nuclear magnetic resonance spectra of 2-acetamido-2-deoxy-D-hexoses and some specifically deuterated, 0-acetylated and phosphorylated derivatives. Canadian Journal of Chemistry 51:3812–3819
    [Google Scholar]
  6. Dische Z. 1947; A new specific color reaction of hexuronic acids. Journal of Biological Chemistry 167:189–198
    [Google Scholar]
  7. Dixon J. S., Lipkin D. 1954; Spectrophotometric determination of vicinal glycols. Application to the determination of ribofuranosides. Analytical Chemistry 26:1092–1093
    [Google Scholar]
  8. Donachie W., Gilmour N. J. L, Poxton I. R. 1982; Cell surface antigens of Pasteurella haemolytica : characterization of the protective antigen in a mouse model. Society for General Microbiology Quarterly 9:Ml3
    [Google Scholar]
  9. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for the determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  10. Fraser J., Lairds S., Gilmour N. J. L. 1982; A new serotype (biotype T) of Pasteurella haemolytica. Research in Veterinary Science 32:127–128
    [Google Scholar]
  11. Gardell S., Heijkenskjold F., Rochnorlund A. 1950; Oxidation of glucosamine and galactosamine with ninhydrin to arabinose and lyxose and their identification with paper chromatography. Acta chemica scandinavica 4:970
    [Google Scholar]
  12. Gilmour N. J. L, Martin W. B., Sharp J. M., Thompson D. A., Wells P. W. 1979; The development of vaccines against pasteurellosis in sheep. Veterinary Record 111:15
    [Google Scholar]
  13. Gorin P. A. J. 1981; Carbon-13 nuclear magnetic resonance spectroscopy of polysaccharides. Advances in Carbohydrate Chemistry and Biochemistry 38:13–104
    [Google Scholar]
  14. Gotschlich E. C., Rey M., Etienne J., Sanborn W. R., Priau R., Cvjetanovic B. 1972; The immunological responses observed in field studies in Africa with Group A meningococcal vaccine. Progress in Immunobiological Standardization 5:485–491
    [Google Scholar]
  15. Goustin A. S., Krick T. P., Anderson J. S. 1983; Use of proton n.m.r. spectroscopy for detection of 2-acetamido-2-deoxy-d-mannose- and 2-acetamido-2-deoxy-d-mannuronate-containing carbohydrates. Carbohydrate Research 119:258–262
    [Google Scholar]
  16. Hansen S. 1975; Thin layer chromatographic method for the identification of mono-, di- and trisaccharides. Journal of Chromatography 107:224–226
    [Google Scholar]
  17. Hestrin S. 1949; The reaction of acetylcholine and other carboxylic acid derivatives with hydroxyl-amine, and its analytical application. Journal of Biological Chemistry 180:249–261
    [Google Scholar]
  18. Horton D., Philips K. D., Defaye J. 1972; The nitrous acid deamination of 2-amino-2-deoxy-d-mannose hydrochloride to d-glucose. Carbohydrate Research 21:417–419
    [Google Scholar]
  19. Jansson P. E., Lindberg B., Lindquist U. 1981; Structural studies of the capsular polysaccharides from Streptococcus pneumoniae type 4. Carbohydrate Research 95:73–80
    [Google Scholar]
  20. Johnson S. D., Lacher K. P., Anderson J. S. 1981; Carbon-13 nuclear magnetic resonance spectroscopic study of the teichuronic acid from Micrococcus luteus cell walls. Comparison of the polysaccharide isolated from cells with that synthesised in vitro. Biochemistry 20:4781–4785
    [Google Scholar]
  21. Kenne L., Lindberg B. 1980; N-Deacetylation of polysaccharides. Methods in Carbohydrate Chemistry 8:295–296
    [Google Scholar]
  22. Lanesmith R., Gilkerson E. 1979; Quantitation of glycosaminoglycan hexosamine using 3-methyl-2-benzothiazolone hydrazone hydrochloride. Analytical Biochemistry 98:478–480
    [Google Scholar]
  23. Liau D. F., Melly M. A., Hash J. H. 1974; Surface polysaccharide from Staphylococcus aureus M that contains taurine, d-aminogalacturonic acid, and d-fucosamine. Journal of Bacteriology 119:913–922
    [Google Scholar]
  24. Lindberg B., Lonngren G. 1978; Methylation analysis of complex carbohydrates: general procedure and application for sequence analysis. Methods in Enzymology 50:3–33
    [Google Scholar]
  25. Mikami T., Nagase T., Matsumoto T., Suzuki S., Suzuki M. 1982; Gelation of Limulus amoebocyte lysate by simple polysaccharides. Microbiology and Immunology 26:403–409
    [Google Scholar]
  26. Morris G. A., Freeman R. 1979; Enhancement of nuclear magnetic resonance signals by polarization transfer. Journal of the American Chemistry Society 101:760–762
    [Google Scholar]
  27. Nagy L. K., Penn C. W. 1974; Protective antigens in bovine pasteurellosis. Developments in Biological Standardization 26:25–32
    [Google Scholar]
  28. Penn C. W., Nagy L. K. 1976; Isolation of a protective, non toxic capsular antigen from Pasteurella multocida, types B and E. Research in Veterinary Science 20:90–96
    [Google Scholar]
  29. Perry M. B., Daoust V., Carlo D. J. 1981; The specific capsular polysaccharide of Streptococcus pneumoniae type 9V. Canadian Journal Biochemistry 59:524–533
    [Google Scholar]
  30. Roe J. H. 1955; The determination of sugar in blood and spinal fluid with anthrone reagent. Journal of Biological Chemistry 212:335–343
    [Google Scholar]
  31. Spiro R. G. 1966; Analysis of sugars found in glycoproteins. Methods in Enzymology 8:43–49
    [Google Scholar]
  32. Stoffyn P. J., Jeanloz R. W. 1954; Identification of amino sugars by paper chromatography. Archives of Biochemistry and Biophysics 52:373–379
    [Google Scholar]
  33. Svennerholm L. 1957; Quantitative estimation of sialic acids II. A colorimetric resorcinol-hydrochloric acid method. Biochimica et biophysica acta 24:604–611
    [Google Scholar]
  34. Taylor R. L., Conrad H. E. 1972; Stoichiometric depolymerization of polyuronides and glycosamino-glycuronans to monosaccharides following reduction of their carbodiimide-activated carboxyl groups. Biochemistry 11:1383–1388
    [Google Scholar]
  35. Tsui F.-P., Boykins R. A., Egan W. 1982; Structural and immunological studies of the Escherichia coli K7 (K56) capsular polysaccharide. Carbohydrate Research 102:263–271
    [Google Scholar]
  36. Tsui F.-P., Schneerson R., Egan W. 1981a; Structural studies of the Haemophilus influenzae type e capsular polysaccharide. Carbohydrate Research 88:85–92
    [Google Scholar]
  37. Tsui F.-P., Schneerson R., Boykins R. A., Karpas A. B., Egan W. 1981b; Structural and immunological studies of the Haemophilus influenzae type d capsular polysaccharide. Carbohydrate Research 97:293–306
    [Google Scholar]
  38. Warren L. 1959; The thiobarbituric acid assay for sialic acids. Journal of Biological Chemistry 234:1971–1975
    [Google Scholar]
  39. Westphal O., Jann K. 1965; Bacterial lipo-polysaccharides. Extraction with phenol-water and further applications of the procedure. Methods in Carbohydrate Chemistry 5:83–92
    [Google Scholar]
  40. Wu T. C. M, Park J. T. 1971; Chemical characterization of a new surface antigenic polysaccharide from a mutant of Staphylococcus aureus. Journal of Bacteriology 108:874–884
    [Google Scholar]
  41. Yoneyama T., Koike Y., Arakawa H., Yokoyama K., Sasaki Y., Kawamura T., Araki Y., Ito E., Takao S. 1982; Distribution of mannosamine and mannosaminuronic acid among cell walls of Bacillus species. Journal of Bacteriology 149:15–21
    [Google Scholar]
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