1887

Abstract

The serotype-specific capsular polysaccharide from two strains of serotype A1 organisms was purified and characterized by chemical analysis and NMR spectroscopy. The polymer has the structure →3)--(2-acetamido-2-deoxy-4--acetyl-β--mannopyranosyluronic acid)-(1→4)--(2-acetamido-2-deoxy-β--mannopyranose)-(1→. The polysaccharide was immunogenic (able to evoke production of antibodies) for sheep but not for rabbits. Immuno electron-microscopy studies using the Protein A-gold technique showed the polysaccharide to be peripherally located on the bacterial surface. Reduction, oxidation and de--acetylation of the polymer did not appear to alter its immunological precipitability with specific antiserum, but all three treatments destroyed its ability to adhere to sheep erythrocytes at neutral pH. De--acetylation of the polymer destroyed both immunological precipitability and erythrocyte adherence.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-9-2415
1984-09-01
2019-10-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-9-2415
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error