1887

Abstract

-Acetylglucosaminidase has been purified from the walls of 168 and compared with the other known autolysin, -acetylmuramyl--alanine amidase (amidase). The -acetylglucosaminidase was a dimer in LiCl buffers with a sub-unit molecular weight of 90000 and a pH optimum of about 5·0. It was very sensitive to proteolytic enzymes and was critically activated by 0·1 to 0·2 -LiCl. It was insoluble in concentrations of LiCl lower than 0·05 to 0·1 . It was less strongly bound to walls than was the amidase, which was a monomer of molecular weight 30000 to 40000 in LiCl buffers. The -acetylglucosaminidase is an endoenzyme and showed no exo-activity. Lysozyme-like enzyme (muramidase) activity was undetectable in the wall extracts examined.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-9-2395
1984-09-01
2021-07-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/9/mic-130-9-2395.html?itemId=/content/journal/micro/10.1099/00221287-130-9-2395&mimeType=html&fmt=ahah

References

  1. Brown W. C., Young F. E. 1970; Dynamic interactions between cell wall polymers, extracellular proteases and autolytic enzymes. Biochemical and Biophysical Research Communications 38:564–568
    [Google Scholar]
  2. Ensign J. C., Wolfe R. S. 1966; Characterization of a small proteolytic enzyme which lyses bacterial cell walls. Journal of Bacteriology 91:524–534
    [Google Scholar]
  3. Fein J. E., Rogers H. J. 1976; Autolytic enzyme- deficient mutants of Bacillus subtilis. . Journal of Bacteriology 127:1427–1442
    [Google Scholar]
  4. Fischer W., Koch H. U., Rosel P., Fiedler F. 1980; Alanine ester-containing native lipoteichoic acids do not act as lipoteichoic acid carrier. Journal of Biological Chemistry 255:4557–4562
    [Google Scholar]
  5. Fischer W., Rosel P., Koch H. U. 1981; Effect of alanine ester substitution and other structural features of lipoteichoic acids on their inhibitory activity against autolysins of Staphylococcus aureus. . Journal of Bacteriology 146:467–475
    [Google Scholar]
  6. Forsberg C., Rogers H. J. 1971; Autolytic enzymes in growth of bacteria. Nature; London: 229272–273
    [Google Scholar]
  7. Herbold D. R., Glaser L. 1975; The purification of Bacillus subtilis autolytic amidase. Journal of Bacteriological Chemistry 250:1676–1682
    [Google Scholar]
  8. Hughes R. C. 1968; The cell wall of Bacillus licheniformis NCTC 6346: isolation of low molecular weight fragments from the soluble mucopeptide. Biochemical Journal 106:49–59
    [Google Scholar]
  9. Hughes C. R., Tanner P. J. 1968; The action of dilute alkali on some bacterial cell walls. Biochemical and Biophysical Research Communications 33:22–28
    [Google Scholar]
  10. Hughes R.C, Thaurman P. F., Stokes E. 1975; Estimate of the porosity of Bacillus licheniformis and Bacillus subtilis cell walls. Zeitschrift für Immunitdts-forschung 149:126–135
    [Google Scholar]
  11. Jensen H. B., Kleppe K. 1972; Studies on T4 lysozyme affinity for chitin and the use of chitin in the purification of the enzyme. European Journal of Biochemistry 26:305–315
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  13. Luria S. E., Burroughs J. W. 1957; Hybridisation between Escherichia coli and Shigella. . Journal of Bacteriology 74:461–476
    [Google Scholar]
  14. Merkeschlager M., Schlossmann K., Kurz W. 1957; Ein mechanischer Zell-homogenisator und seine Anwendbarkeit auf biologische Probleme. Biochemiche Zeitschrift 329:332
    [Google Scholar]
  15. Okada S., Itahata S. 1973; Purification and properties of bacterial lysozyme. Journal of Fermentation Technology 51:705–712
    [Google Scholar]
  16. Ortiz J. M., Gillespie J. B. 1972; A exO-β-N-acetylglucosaminidase from bacillus subtilis: extraction and purification. Biochimica et biophysica acta 289:174–186
    [Google Scholar]
  17. Pollock M. R. 1962; Exoenzymes. In: The Bacteria, 4 pp. 121–178 Gunsalus I. C., Stanier R. Y. Edited by New York: Academic Press;
    [Google Scholar]
  18. Rogers H. J., Perkins H. R., Ward J. B. 1980 Cell Walls and Membranes p. 51 London: Chapman & Hall;
    [Google Scholar]
  19. Rogers H. J., Pooley H. M., Thurman P. F., Taylor C. 1974; Wall and membrane growth in bacilli and their mutants. Annales de microbiologie 125B:135–147
    [Google Scholar]
  20. Rogers H. J., Thurman P. F., Burdett I. D. J. 1983; The bactericidal action of β-lactam antibiotics on an autolysin-deficient strain of Bacillus subtilis. . Journal of General Microbiology 129:465–478
    [Google Scholar]
  21. Takahara Y., Machigaki E., Maruo S. 1974; General properties of endo-N-acetylmuramidase of Bacillus subtilis T-25. Agricultural and Biological Chemistry 38:2357–2365
    [Google Scholar]
  22. Taylor C., Rogers H. J., Ward J. B. 1980; The autolytic endo-β-N-acetylglucosaminidase of Bacillus subtilis: initial characterisation. Society for General Microbiology Quarterly 7:73–74
    [Google Scholar]
  23. Williamson R., Ward J. B. 1981; Deficiency of autolytic activity in Bacillus subtilis and Streptococcus pneumoniae is associated with a decreased permeability of the wall. Journal of General Microbiology 125:325–334
    [Google Scholar]
  24. Young F. E. 1966; Autolytic enzyme associated with cell walls of Bacillus subtilis. . Journal of Biological Chemistry 241:3462–3467
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-9-2395
Loading
/content/journal/micro/10.1099/00221287-130-9-2395
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error