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Abstract
Protoplasts isolated from Saprolegnia monoica were used to study β-glucan synthases and polysaccharide synthesis during wall regeneration. (1→4)-β- and (1→3)-β-glucan synthase activities of plasma membranes and internal membranes increased during regeneration. Within minutes of cultivation, protoplasts transferred radioactivity from [14C]glucose to cellulose and other cell wall polymers. UDP[14C]glucose did not serve as substrate. Cellulose microfibrils were produced from the beginning of regeneration. The early phases of regeneration are realized by plasma membrane enzymes having remained intact during protoplast isolation and independently of the Golgi apparatus.
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Keyword(s):
Con-A, concanavalin A
,
IMF, internal membrane fraction
and
PMF, plasma membrane fraction
© Society for General Microbiology, 1984