1887

Abstract

Protoplasts isolated from were used to study -glucan synthases and polysaccharide synthesis during wall regeneration. (1→4)-- and (1→3)--glucan synthase activities of plasma membranes and internal membranes increased during regeneration. Within minutes of cultivation, protoplasts transferred radioactivity from [C]glucose to cellulose and other cell wall polymers. UDP[C]glucose did not serve as substrate. Cellulose microfibrils were produced from the beginning of regeneration. The early phases of regeneration are realized by plasma membrane enzymes having remained intact during protoplast isolation and independently of the Golgi apparatus.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-9-2367
1984-09-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/9/mic-130-9-2367.html?itemId=/content/journal/micro/10.1099/00221287-130-9-2367&mimeType=html&fmt=ahah

References

  1. Burnett J. H. 1976 Fundamentals of Mycology, 2nd edn. . London: Edward Arnold;
    [Google Scholar]
  2. Févre M. 1979a; Glucanases, glucan synthases and wall growth in Saprolegnia monoica. . In Fungal Walls and Hyphal Growth pp. 225–263 Burnett J. H., Trinci A. P. J. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
  3. Févre M. 1979b; Digitonin solubilization and protease stimulation of β-glucan synthetases of Saprolegnia. . Zeitschrift für Pflanzenphysiologie 95:129–140
    [Google Scholar]
  4. Févre M., Rougier M. 1981; β1–3 and β1–4 glucan synthesis by membrane fractions from the fungus Saprolegnia. . Planta 151:232–241
    [Google Scholar]
  5. Gaugy D., Févre M. 1982; Protoplast production from Saprolegnia monoica. . Microbios 34:89–98
    [Google Scholar]
  6. Girard D., Févre M. 1984a; β1–4 and β1–3 glucan synthetases are associated with the plasma membrane of the fungus Saprolegnia. . Planta 160:400–406
    [Google Scholar]
  7. Girard D., Févre M. 1984b; Distribution of (1→3)-β- and (l→4)-β-glucan synthases along the hyphae of Saprolegnia. . Journal of General Microbiology 130:1557–1562
    [Google Scholar]
  8. Klein A. S., Montezinos D., Delmer D. P. 1981; Cellulose and 1→3β glucan synthesis during the early stages of wall regeneration in soybean protoplasts. Planta 152:105–114
    [Google Scholar]
  9. Machlis L. 1953; Growth and nutrition of water- molds in the subgenus Euallomyces. II. Optimal composition of the minimal medium. American Journal of Botany 40:449–460
    [Google Scholar]
  10. Peberdy J. F. 1979; Wall biogenesis by protoplasts. In Fungal Walls and Hyphal Growth pp. 49–70 Burnett J. H., Trinci A. P. J. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
  11. Updegraff D. M. 1969; Semi-micro determination of cellulose in biological materials. Analytical Biochemistry 32:420–424
    [Google Scholar]
  12. Wessels J. G. H, Valk P., van Der, de Vries O. M. H. 1976; Wall synthesis by fungal protoplasts. In Microbial and Plant Protoplasts pp. 267–281 Peberdy J. F., Rose A. H., Rogers H. J., Cocking C. C. Edited by London: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-9-2367
Loading
/content/journal/micro/10.1099/00221287-130-9-2367
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error